ارزیابی فیتوشیمیایی، آنتی¬اکسیدانی و ضدآلزایمری نانوذرات نقره سنتز شده با عصاره آبی گیاه خارشتر (Alhagi persarum)
محورهای موضوعی : بیوشیمی
نیلوفر واحدپوری
1
,
امیر آراسته
2
1 - گروه زیست شناسی، واحد رشت، دانشگاه آزاد اسلامی، رشت، ایران
2 - عضو هیئت عملی رشت، دانشگاه آزاد اسلامی واحد رشت، دانشکده ی علوم پایه، گروه میکروبیولوژی
کلید واژه: Alhagi persarum, آنتی اکسیدان, استیل کولین استراز, بیماری آلزایمر, نانوذره,
چکیده مقاله :
گیاه دارویی خارشتر (Alhagi persarum) از گیاهان مقاوم مناطق خشک است که جایگاه ویژه¬ای در طب سنتی ایرانی دارد. هدف از این پژوهش بررسی فیتوشیمیایی، آنتی¬اکسیدانی و ضدآلزایمری نانوذرات نقره سنتز شده با عصاره آبی خارشتر بوده است. ترکیبات موجود در عصاره با گاز کروماتوگرافی جرمی و میزان مهار رادیکال¬های آزاد با آزمون DPPH و میزان مهار آنزیم استیل¬کولین استراز با روش طیف¬سنجی تعیین شد. مهار تولید رشته¬های آمیلوئیدی با طیف¬سنجی فلورسانس بررسی و با میکروسکوپ الکترونی گزاره تائید گردید. تولید نانوذرات نقره با طیف¬سنجی مرئی و مادون قرمز تائید شد و ساختار آن با روش¬ پراکندگی نور دینامیکی (DLS)، پتانسیل زتا، میکروسکوپ الکترونی روبشی (SEM) و طیف¬سنجی پراش پرتو ایکس (XRD) بررسی شد. بیش¬ترین ترکیبات موجود در عصاره Thiophen، Octadecadienoic acid و Inositol بود. با افزایش غلظت نانوذرات، فعالیت آنتی¬اکسیدانی افزایش یافت و بیش¬ترین اثرات مهاری بر آنزیم استیل¬کولین استراز و همچنین بیش¬ترین اثرات مهاری بر تولید رشته¬های آمیلوئیدی در غلظت¬های کم نانوذرات مشاهده گردید. عصاره گیاه A. persarum با اثرات آنتی-اکسیدانی و مهاری که بر آنزیم استیل کولین استراز دارد، می¬تواند به¬عنوان یک ترکیب مکمل غذایی مناسب برای کاهش عوارض بالینی در مبتلایان به آلزایمر مورد استفاده قرار گیرد.
Abstract Alhagi persarum is one of the resistant plants of dry regions, which has a special place in Iranian traditional medicine. The aim of this research was to investigate the phytochemical, antioxidant and anti-Alzheimer properties of silver nanoparticles synthesized with the aqueous extract of A. persarum. The compounds in the extract were determined by GC-MS and the inhibition rate of free radicals was determined by DPPH test and the inhibition rate of acetylcholinesterase enzyme was determined by spectrometry method. Inhibition of the production of amyloid fibrils was investigated by fluorescence spectroscopy and confirmed by electron microscopy. The production of silver nanoparticles was confirmed by visible and infrared spectroscopy, and its structure was investigated by dynamic light scattering (DLS), zeta potential, scanning electron microscopy (SEM) and X-ray diffraction spectroscopy (XRD). The most compounds in the extract were Thiophen, Octadecadienoic acid and Inositol. By increasing the concentration of nanoparticles, the antioxidant activity increased and the most inhibitory effects on acetyl-cholinesterase enzyme as well as the most inhibitory effects on the production of amyloid fibrils were observed at low concentrations of nanoparticles. A. persarum plant extract, with its antioxidant and inhibitory effects on acetylcholinesterase enzyme, can be used as a suitable food supplement to reduce clinical complications in Alzheimer's patients.
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