Effect of Protein Levels and Rumen Protected Glutamine Supplementation on Blood Metabolites, Thyroid Hormones, and Redox Status of Heat Stressed Fattening Lambs
محورهای موضوعی : Camel
M. Feyz
1
(Department of Animal Science, Faculty of Animal Science and Fishery, Sari Agricultural Science and Natural Resources University, Sari, Iran)
A. Teimouri Yansari
2
(Department of Animal Science, Faculty of Animal Science and Fishery, Sari Agricultural Science and Natural Resources University, Sari, Iran)
Y. Chashnidel
3
(Department of Animal Science, Faculty of Animal Science and Fishery, Sari Agricultural Science and Natural Resources University, Sari, Iran)
E. Dirandeh
4
(Department of Animal Science, Faculty of Animal Science and Fishery, Sari Agricultural Science and Natural Resources University, Sari, Iran)
کلید واژه: antioxidant enzyme, blood metabolite, temperature humidity index, triiodothyron-ine,
چکیده مقاله :
High ambient temperatures have a negative effect on the production and health of animals. Severe heat stress may lead to changes in the secretion of hypothalamus and pituitary gland hormones, which alter hormonal responses and metabolism. The aim of this study was to determine the effect of protein levels and rumen protected glutamine supplementation on blood metabolites, thyroid hormones concentrations, and redox status of fattening heat stressed lambs. Using sixteen Afshari male lambs (aged 3-4 months) over 45 days, a 2 × 2 factorial were designed to study the effects of two levels of protein (equal and 10% higher than requirement) and glutamine (0.0 and 0.2 g/kg body weight) in four experimental rations. Results showed the glutamine supplementation increased triiodothyronine and thyroxine concentrations; and conversely decreased aspartate transaminase, non-esterified fatty acids, and cortisol concentrations. Increased protein levels at the beginning of fattening did not affect triiodothyronine, thyroxine, aspartate transaminase, alanine transaminase, and non-esterified fatty acids concentrations. Interaction of glutamine and protein levels significantly decreased cortisol concentrations. Glutamine supplementation increased levels of superoxide dismutase and glutathione peroxidase, and decreased nitric oxide. The greater protein levels significantly decreased superoxide dismutase and glutathione peroxidase on day 30, total antioxidant status concentration on day 15 and 45, and increased nitric oxide concentration on day 15 of fattening. It can be concluded that glutamine supplementation improves redox status of heat stressed lambs during the fattening period but increase protein levels alone did not have this effect.
Adams F. and Ohene-Yankyera K. (2014). Socio-economic characteristics of subsistent small ruminant farmers in three regions of Northern Ghana. Asian J. Appl. Sci. Eng. 3, 93-106.
Afsal A., Sejian V., Bagath M., Krishnan G., Devaraj C. and Bhatta R. (2018). Heat stress and livestock adaptation: Neuro-endocrine regulation. Int. J. Vet. Anim. Med. 1(2), 108-119.
Aizawa S., Sakai T. and Sakata I. (2012). Glutamine and glutamic acid enhance thyroid-stimulating hormone b subunit mRNA expression in the rat pars tuberalis. J. Endocrinol. 212, 383-394
Alameen A.O. and Abdelatif A.M. (2012). Endocrine responses of crossbred dairy cows in relation to pregnancy and season under tropical conditions. American Eurasian J. Agric. Environ. Sci. 12, 1065-1074.
Banerjee D., Upadhyay R.C., Chaudhary U.B., Kumar R., Singh S., Das A.T.K. and De S. (2015). Seasonal variations in physio-biochemical profiles of Indian goats in the paradigm of hot and cold climate. Biol. Rhythm Res. 46, 221-236.
Branzk N., Lubojemska A., Hardison S.E., Wang Q., Gutierrez M.G., Brown G.D. and Papayannopoulos V. (2014). Neutrophils sense microbe size and selectively release neutrophil extracellular traps in response to large pathogens. Nat. Immunol. 15, 1017-1025.
Chaudhary S.S., Singh V.K., Upadhyay R.C., Puri G., Odedara A.B. and Patel P.A. (2015). Evaluation of physiological and biochemical responses in different seasons in Surti buffaloes. Vet. Worl. 8(6), 727-731.
Cichosz G. and Czeczot H. (2013). Controversions around diet proteins. Polish Med. J. 35(210), 397-401
Cong X., Zhang Q., Li H., Jiang Z., Cao R., Gao S. and Tian W. (2017). Puerarin ameliorates heat stress–induced oxidative damage and apoptosis in bovine Sertoli cells by suppressing ROS production and upregulating Hsp72 expression. J. Theriogenol. 88, 215-227.
Cortas N. and Wakid N. (1990). Determination of inorganic nitrate in serum and urine by a kinetic cadmium-reduction method. Clin. Chem. 36(8), 1440-1443.
Cruzat V., Macedo Rogero M., Noel Keane K., Curi R. and Newsholme P. (2018). Glutamine: Metabolism and immune function, supplementation and clinical translation. Nutrients. 10, 1564.
Deger Y., Ertekin A., Deger S. and Mert H. (2008). Lipid peroxidation and antioxidant potential of sheep liver infected naturally with distomatosis. Turkiye Parazitol. Derg. 32(1), 23-26.
Fekete C. and Lechan R.M. (2013). Central regulation of hypothalamic-pituitary-thyroid axis under physiological and pathophysiological conditions. Endocr Rev. 35, 159-194.
Gonzales R., Auclair C., Voisin E., Gautero H., Dhermy D. and Boivin P. (1984). Superoxide dismutase, catalase and glutathione peroxydase in red blood cells from patients with malignant diseases. Cancer Res. 44, 4137-4139.
Joy A., Pragna P., Archana P.R., Sejian P.R. and Bagath M. (2016). Significance of metabolic response in livestock for adapting to heat stress challenges. Asian J. Anim. Sci. 10, 224-234.
Karami S., Kashef M. and Mehri Alvar Y. (2014). Protective effect of glutamine by the expression of HSP70 and reduction of cortisol on exercise induced stress. J. Arak Univ. Med. Sci. 17(91), 65-73.
McManus C.M., Paludo G.R., Louvandini H., Gugel R., Sasaki L.C.B. and Paiva S.R. (2009). Heat tolerance in Brazilian sheep: physiological and blood parameters. Trop. Anim. Health Prod. 41, 95-101.
Mills E.L., Kelly B. and O’Neill L.A. (2017). Mitochondria are the powerhouses of immunity. Nat. Immunol. 18, 488-498.
Moradi M., Chashnidel Y., Teimouri Yansari A. and Dirandeh E. (2018). Effects of increasing level of metabolizable energy and protein on feed intake, nutrient digestibility, performance and blood metabolites of Zell pregnant ewes at late gestation. Res. Anim. Prod. 9(22), 60-71.
Mullur R., Liu Y.Y. and Brent G.A. (2014). Thyroid hormone regulation of metabolism. Physiol. Rev. 94, 355-382.
Nazari A., Dirandeh E., Ansari-Pirsaraei Z. and Deldar H. (2019). Antioxidant levels, copper and zinc concentrations were associated with postpartum luteal activity, pregnancy loss and pregnancy status in Holstein dairy cows. Theriogenology. 15, 133:97-103.
Nemati M., Menatian S., Joz Ghasemi S., Hooshmandfar R., Taheri M. and Saifi T. (2018). Effect of protected-glutamine supplementation on performance, milk composition and some blood metabolites in fresh Holstein cows. Iranian J. Vet. Res. 19(3), 225-228.
Nisar A., Sultana M. and Ashraf H. (2013). Oxidative stress-threat to animal health and production. Int. J. Livest. Res. 3, 76-83.
Niyas P.A.A., Chaidanya K., Shaji S., Sejian V. and Bhatta R. (2015). Adaptation of livestock to environmental challenges. J. Vet. Sci. Med. Diagn. 4, 3-13.
Okoruwa M.I. (2014). Effect of heat stress on thermoregulatory, live bodyweight and physiological responses of dwarf goats in southern Nigeria. European Sci. J. 10, 255-264.
Ou C.Y., He Y.H., Sun Y., Yang L., Shi W.X. and Li S. (2019). Effects of sub-acute manganese exposure on thyroid hormone and glutamine (Gln)/glutamate (Glu)-γaminobutyric acid (GABA) cycle in serum of rats. Int. J. Environ. Res. Public Health. 16(12), 2157-2168.
Pithon-Curi T.C., De Melo M.P. and Curi R. (2004). Glucose and glutamine utilization by rat lymphocytes, monocytes and neutrophils in culture: A comparative study. Cell Biochem. Funct. 22, 321-326.
Rathwa S.D., Vasava A.A., Pathan M.M., Madhira S.P., Patel Y.G. and Pande A.M. (2017). Effect of season on physiological, biochemical, hormonal, and oxidative stress parameters of indigenous sheep. Vet. World. 10(6), 650-654.
SAS Institute. (2004). SAS®/STAT Software, Release 9.4. SAS Institute, Inc., Cary, NC. USA.
Sejian V., Maurya V.P. and Naqvi S.M. (2010). Adaptive capability as indicated by endocrine and biochemical responses of Malpura ewes subjected to combined stresses (thermal and nutritional) in a semi-arid tropical environment. Int. J. Biometeorol. 54(6), 653-661.
Shaji S., Sejian V., Bagath M., Manjunathareddy G.B., Kurien E.K., Varma G. and Bhatta R. (2017). Summer season related heat and nutritional stresses on the adaptive capability of goats based on blood biochemical response and hepatic HSP70 gene expression. Biol. Rhythm Res. 48, 65-83.
Stachowicz M. and Lebiedzinska A. (2016). The effect of diet components on the level of cortisol. European Food Res. Technol. 242, 2001-2009.
Tanha T., Amanlou H., Chamani M., Ebrahimnezhad Y., Salamatdost R., Maheri N. and Fathi M. (2011). Effect of glutamine enhancement on oxidative stress and reproduction in Holstein dairy cows during transition period. J. Anim. Vet. Adv. 10(21), 2838-2845.
