اثر اولئوروپین بر میزان گلوکز خون و بیان ژن پیروات کیناز در موش های صحرایی نر دیابتی شده با استرپتوزوتوسین
اثر اولئوروپین بر گلوکز خون و ژن پیروات کیناز در موش های صحرایی دیابتی
محورهای موضوعی : نشانگر های زیستی پلاسما
اکرم اسدی
1
,
مریم رفیعی راد
2
,
آمنه جاوید
3
1 - علم و هنر یزد
2 - گروه زیست شناسی دانشگاه آزاد اسلامی واحد ایذه
3 - گروه زیست شناسی دانشکده مهندسی و علوم دانشگاه علم و هنر یزد
کلید واژه: دیابت, اولئوروپین, پیروات کیناز, موش صحرایی,
چکیده مقاله :
زمینه و هدف: با توجه به خواص آنتی اکسیدانی اولئوروپین و نقش آنتی اکسیدان ها در بهبود دیابت، این مطالعه با هـدف بررسی اثر اولئوروپین بر میزان گلوکز خون و بیان ژن پیروات کیناز در موش های صحرایی نر دیابتی انجام شد. مواد و روش ها : با تزریق استرپتوزوتوسین (mg/kg60) در موش هاي صحرایی نر (250-200گرم) دیابت ایجـاد شـد. ح پس از تزریق داخل صفاقی STZ، حیوانات دارای قند خون بالای 200mg/dL، دیابتی تلقی شدند. گروه‌های کنترل و گروه دیابتی فقط سالین و گروه‌های دیابتی درمان شده به مدت چهار هفته روزانه بصورت گاواژ اولئوروپین mg/kg 10،20دریافت کردند. سپس رت ها با دارو بی هوش و تشریح شدند. بافت کبد جداسازی و برای بررسی بیان ژن پیروات کیناز به روش Real-time PCR استفاده شد. تجزیه و تحلیل داده‌ها با استفاده از آزمون توکی در سطح P<0.05 انجام شد. نتایج : نتایج نشان داد در گروه دیابتی دریافت کننده دوزهای mg/kg 20و10 اولئوروپین نسبت به گروه دیابتی به طور معناداری کاهش یافت. بررسی بیان ژن پیروات کیناز نیز نـشان داد در گروه دیابتی ﻧﺴﺒﺖ ﺑﻪ گروه کنترل ﺳﺎﻟﻢ ﺩﺍﺭﺍﻱ کاهش ﺑﻮﺩ ﺍﻣﺎ ﺍﻳﻦ کاهش ﺍﺯ ﻧﻈﺮ ﺁﻣﺎﺭﻱ ﺩﺭ ﺣﺪ ﻣﻌﻨﻲﺩﺍﺭﻱ ﺑﻴﺎﻥ ﻧﺸﺪ. از طرفی ﺩﺭ گروه دیابتی تحت درمان با دوز های mg/kg 20 و 10 اولئوروپین میزان بیان ژن پیروات کیناز نسبت به گروه دیابتی به طور معناداری افزایش داشته است. نتیجه گیری: نتایج تحقیق حاضر نشان می دهد که احتمالا تجویز اولئوروپین توانسته از طریق تغییر میزان بیان ژن پیرووات کیناز باعث کاهش گلوکز خون شود
Background & Aim: Considering the antioxidant properties of oleuropein and the role of antioxidants in improving diabetes, this study was conducted with the aim of investigating the effect of oleuropein on blood glucose levels and pyruvate kinase gene expression in diabetic male rats. Materials & Methods: 40 rats aged 2-3 months (weighing 200-250 grams) were divided into four groups of ten. By injecting streptozotocin (60 mg/kg), type 1 diabetes was induced in male rats. After intraperitoneal injection of STZ, animals with blood glucose above 200 mg/kg were considered diabetic. The control groups and the diabetic group only received saline and the treated diabetic groups received 10, 20 mg/kg oleuropein daily by gavage for four weeks. At the end of the experiment period, all the rats were dissected after anesthesia with ether by drawing blood from the heart and liver tissue was isolated and used to check the expression of pyruvate kinase gene by Real-time PCR method. Data analysis was performed using Tukey's test at P<0.05 level. Results: The results showed that in the diabetic group receiving doses of 10 and 20 mg/kg of oleuropein, the blood glucose level decreased significantly compared to the diabetic group. Examining the expression of pyruvate kinase gene also showed that there was a decrease in the diabetic group compared to the healthy control group, but this decrease was not statistically significant. On the other hand, in the diabetic group treated with 20 and 10 mg/kg doses of oleuropein, pyruvate kinase gene expression increased significantly compared to the diabetic group. Conclusion: The results of the present study showed that the administration of oleuropein has been able to reduce blood glucose by changing the expression of the pyruvate kinase gene.
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