Removal of Malachite Green by Using Immobilized Glucose Oxidase Onto Silica Nanostructure-Coated Silver Metal-Foam

Subject Areas : Journal of Nanoanalysis

Mansour Hemmati ¹ , Mohammad Barkhi ² , Hadi Baharifar ³ , Kamyar Khoshnevisan ⁴

1 - Nano Pooshesh Felez Co., University of Tehran science and Technology Park, Tehran, Iran
2 - University of Applied Science and Technology (UAST), Zar Branch, Karaj, Iran
3 - Department of Medical Nanotechnology, Applied Biophotonics Research Center, Science and Research Branch, Islamic Azad University, Tehran, Iran
4 - Nano Pooshesh Felez Co., University of Tehran science and Technology Park, Tehran, Iran Biosensor Research Center, Endocrinology and Metabolism Molecular-Cellular Sciences Institute, Tehran University of Medical Sciences, Tehran, Iran Endocrinology and Metabolism Research Center, Endocrinology and Metabolism Research Institute, Tehran University of Medical Sciences, Tehran, Iran

Received: 2017-04-17 Accepted : 2017-04-17 Published : 2017-09-01

Keywords: Malachite green, Enzyme activity, Enzyme immobilization, Glucose oxidase, Silver metal foam,

Abstract :

Enzymes Immobilization onto different types of the substrate could be helpful in various applicationsof biomedical devices and biosensors. Enzyme activity and stability could be affected by support andmethod of immobilization. In this study, the silver metal foam was successfully synthesized by thesoft-shell method and then was coated with silica. Then, glucose oxidase (GOx) immobilized on noncoated,and silica-coated silver metal foam and removal of malachite green was investigated. FourierTransform Infrared Spectroscopy (FT-IR) and scanning electron microscopy (SEM) results confirmedthat the enzyme was attached to support surface. The maximum immobilized enzyme activity wasabout 118.980 U/grSupport at 40°C. The removal of malachite green showed the indirect relationwith dye concentration confirmed by decolorization assay. The high activity, thermal stability, andreusability of immobilized glucose oxidase onto the silver metal foam in comparison to free enzymeintroduce the capability of the biological system for removal of malachite green in the industry.