A novel soft polycaprolactone-alginate nanofiber plasma-modified with sufficient cell attachment for tissue engineering
Subject Areas : Journal of NanoanalysisElham Azizifard 1 , َAzadeh Asefnejad 2 , Sedigheh Joughehdoust 3 , Hadi Baharifar 4
1 - Department of Biomedical Engineering, Science and Research Branch, Islamic Azad University, Tehran, Iran
2 - Department of Biomedical Engineering, Science and Research Branch, Islamic Azad University, Tehran, Iran
3 - Department of Biomedical Engineering, Science and Research Branch, Islamic Azad University, Tehran, Iran
4 - Department of Biomedical Engineering, Science and Research Branch, Islamic Azad University, Tehran, Iran
Keywords: Sodium alginate, Wound, Tissue Engineering, Polycaprolactone, Light microscopy,
Abstract :
Degradable polymers belonging to the aliphatic polyester family are currently the most attractive group of synthetic polymers. Natural and synthetic materials used in tissue engineering scaffolds should have properties such as proper biocompatibility and biodegradability with controllable degradation and adsorption rate. Synthetic polymers provide the mechanical support required by the system and the tensile strength for cell attachment and growth. Compared to synthetic polymers, natural polymers are more compatible and reduce the likelihood of tissue rejection after transplantation. In this article, sodium alginate (SA), polyvinyl alcohol (PVA) and polycaprolactone (PCL) were used to produced porous scaffold. For this purpose, different percentages of SA and PVA were prepared for electrospinning technique. The PCL/80PVA: 20SA scaffold was evaluated by fourier-transform infrared spectroscopy (FTIR), scanning electron microscope (SEM), light microscopy (LM), swelling, biodegradability and biocompatibility analyzes after plasma process. Morphological examination showed the fiber diameter was about 299 nm and the inflation and degradation were reported at 92% and 18%, respectively. The contact angle created is equal to 42 °and the biocompatibility study for the scaffold showed 93% survival rate. The obtained results showed that PCL/80PVA: 20SA scaffold after plasma can be used in tissue engineering.
[1] Khandan, A., Abdellahi, M., Ozada, N., & Ghayour, H. (2016). Study of the bioactivity, wettability and hardness behaviour of the bovine hydroxyapatite-diopside bio-nanocomposite coating. Journal of the Taiwan Institute of Chemical Engineers, 60, 538-546.
[2] Karamian, E., Motamedi, M. R. K., Khandan, A., Soltani, P., & Maghsoudi, S. (2014). An in vitro evaluation of novel NHA/zircon plasma coating on 316L stainless steel dental implant. Progress in Natural Science: Materials International, 24(2), 150-156.
[3] Karamian, E., Abdellahi, M., Khandan, A., & Abdellah, S. (2016). Introducing the fluorine doped natural hydroxyapatite-titania nanobiocomposite ceramic. Journal of Alloys and Compounds, 679, 375-383.
[4] Najafinezhad, A., Abdellahi, M., Ghayour, H., Soheily, A., Chami, A., & Khandan, A. (2017). A comparative study on the synthesis mechanism, bioactivity and mechanical properties of three silicate bioceramics. Materials Science and Engineering: C, 72, 259-267.
[5] Ghayour, H., Abdellahi, M., Ozada, N., Jabbrzare, S., & Khandan, A. (2017). Hyperthermia application of zinc doped nickel ferrite nanoparticles. Journal of Physics and Chemistry of Solids, 111, 464-472.
[6] Kazemi, A., Abdellahi, M., Khajeh-Sharafabadi, A., Khandan, A., & Ozada, N. (2017). Study of in vitro bioactivity and mechanical properties of diopside nano-bioceramic synthesized by a facile method using eggshell as raw material. Materials Science and Engineering: C, 71, 604-610.
[7] Khandan, A., & Ozada, N. (2017). Bredigite-Magnetite (Ca7MgSi4O16-Fe3O4) nanoparticles: A study on their magnetic properties. Journal of Alloys and Compounds, 726, 729-736.
[8] Khandan, A., Jazayeri, H., Fahmy, M. D., & Razavi, M. (2017). Hydrogels: Types, structure, properties, and applications. Biomat Tiss Eng, 4(27), 143-69.
[9] Sharafabadi, A. K., Abdellahi, M., Kazemi, A., Khandan, A., & Ozada, N. (2017). A novel and economical route for synthesizing akermanite (Ca2MgSi2O7) nano-bioceramic. Materials Science and Engineering: C, 71, 1072-1078.
[10] Shayan, A., Abdellahi, M., Shahmohammadian, F., Jabbarzare, S., Khandan, A., & Ghayour, H. (2017). Mechanochemically aided sintering process for the synthesis of barium ferrite: Effect of aluminum substitution on microstructure, magnetic properties and microwave absorption. Journal of Alloys and Compounds, 708, 538-546
[11] Kardan-Halvaei, M., Morovvati, M. R., & Mollaei-Dariani, B. (2020). Crystal plasticity finite element simulation and experimental investigation of the micro-upsetting process of OFHC copper. Journal of Micromechanics and Microengineering, 30(7), 075005.
[12] Huang, Z.M., Zhang, Y.Z., Kotaki, M. and Ramakrishna, S., 2003. A review on polymer nanofibers by electrospinning and their applications in nanocomposites. Composites science and technology, 63(15), pp.2223-2253.
[13] Feng, L., Li, S., Li, H., Zhai, J., Song, Y., Jiang, L. and Zhu, D., 2002. Super‐hydrophobic surface of aligned polyacrylonitrile nanofibers. Angewandte Chemie International Edition, 41(7), pp.1221-1223.
[14] Iimura, K., Oi, T., Suzuki, M. and Hirota, M., 2010. Preparation of silica fibers and non-woven cloth by electrospinning. Advanced Powder Technology, 21(1), pp.64-68.
[15] Li, Z. and C. Wang, 2013. Applications of Electrospun Nanofibers, in One-Dimensional nanostructures. Springer. p. 139-75.
[16] Lucchini, R., Carnelli, D., Gastaldi, D., Shahgholi, M., Contro, R., & Vena, P. (2012). A damage model to simulate nanoindentation tests of lamellar bone at multiple penetration depth. In 6th European Congress on Computational Methods in Applied Sciences and Engineering, ECCOMAS 2012 (pp. 5919-5924).
[17] Mahjoory, M., Shahgholi, M., & Karimipour, A. (2022). The effects of initial temperature and pressure on the mechanical properties of reinforced calcium phosphate cement with magnesium nanoparticles: A molecular dynamics approach. International Communications in Heat and Mass Transfer, 135, 106067.
[18] Talebi, M., Abbasi‐Rad, S., Malekzadeh, M., Shahgholi, M., Ardakani, A. A., Foudeh, K., & Rad, H. S. (2021). Cortical bone mechanical assessment via free water relaxometry at 3 T. Journal of Magnetic Resonance Imaging, 54(6), 1744-1751.
[19] Shahgholi, M., Oliviero, S., Baino, F., Vitale-Brovarone, C., Gastaldi, D., & Vena, P. (2016). Mechanical characterization of glass-ceramic scaffolds at multiple characteristic lengths through nanoindentation. Journal of the European Ceramic Society, 36(9), 2403-2409.
[20] Fada, R., Farhadi Babadi, N., Azimi, R., Karimian, M., & Shahgholi, M. (2021). Mechanical properties improvement and bone regeneration of calcium phosphate bone cement, Polymethyl methacrylate and glass ionomer. Journal of Nanoanalysis, 8(1), 60-79.
[21] Razavi, M., & Khandan, A. (2017). Safety, regulatory issues, long-term biotoxicity, and the processing environment. In Nanobiomaterials Science, Development and Evaluation (pp. 261-279). Woodhead Publishing.
[22] Khandan, A., Ozada, N., & Karamian, E. (2015). Novel microstructure mechanical activated nano composites for tissue engineering applications. J Bioeng Biomed Sci, 5(1), 1.
[23] Ghayour, H., Abdellahi, M., Bahmanpour, M., & Khandan, A. (2016). Simulation of dielectric behavior in RFeO $$ _ {3} $$3 orthoferrite ceramics (R= rare earth metals). Journal of Computational Electronics, 15(4), 1275-1283.
[24] Saeedi, M., Abdellahi, M., Rahimi, A., & Khandan, A. (2016). Preparation and characterization of nanocrystalline barium ferrite ceramic. Functional Materials Letters, 9(05), 1650068.
[25] Khandan, A., Karamian, E., Faghih, M., & Bataille, A. (2014). Formation of AlN Nano Particles Precipitated in St-14 Low Carbon Steel by Micro and Nanoscopic Observations. Journal of Iron and Steel Research International, 21(9), 886-890.
[26] Saeedi, M. R., Morovvati, M. R., & Mollaei-Dariani, B. (2020). Experimental and numerical investigation of impact resistance of aluminum–copper cladded sheets using an energy-based damage model. Journal of the Brazilian Society of Mechanical Sciences and Engineering, 42(6), 1-24.
[27] Shahverdi, S., Hajimiri, M., Esfandiari, M.A., Larijani, B., Atyabi, F., Rajabiani, A., Dehpour, A.R., Gharehaghaji, A.A. and Dinarvand, R., 2014. Fabrication and structure analysis of poly (lactide-co-glycolic acid)/silk fibroin hybrid scaffold for wound dressing applications. International Journal of Pharmaceutics, 473(1-2), pp.345-355.
[28] Ghadirinejad, N., Nejad, M. G., & Alsaadi, N. (2021). A fuzzy logic model and a neuro-fuzzy system development on supercritical CO2 regeneration of Ni/Al2O3 catalysts. Journal of CO2 Utilization, 54, 101706.
[29] Ghasemi, M., Nejad, M. G., & Aghaei, I. (2021). Knowledge management orientation and operational performance relationship in medical tourism (overview of the model performance in the COVID-19 pandemic and post-pandemic era). Health Services Management Research, 34(4), 208-222.
[30] Ghasemi, M., Nejad, M. G., & Bagzibagli, K. (2017). Knowledge management orientation: an innovative perspective to hospital management. Iranian journal of public health, 46(12), 1639.
[31] Arafat, M. T., M. M. Savalani, and I. Gibson. "Improving the mechanical properties in tissue engineered scaffolds." In ASME 2008 International Mechanical Engineering Congress and Exposition, pp. 3-6. American Society of Mechanical Engineers.
[32] Zhang, S., 2003. Fabrication of novel biomaterials through molecular self-assembly. Nature biotechnology, 21(10), pp.1171-1178.
[33] Kazemi, A., Abdellahi, M., Khajeh-Sharafabadi, A., Khandan, A., & Ozada, N. (2017). Study of in vitro bioactivity and mechanical properties of diopside nano-bioceramic synthesized by a facile method using eggshell as raw material. Materials Science and Engineering: C, 71, 604-610.
[34] Morovvati, M. R., & Dariani, B. M. (2017). The effect of annealing on the formability of aluminum 1200 after accumulative roll bonding. Journal of Manufacturing Processes, 30, 241-254.
[35] Rezaei, H., Asefnejad, A., Daliri-Joupari, M., & Joughehdoust, S. (2021). In-vitro cellular and in-vivo investigation of ascorbic acid and β-glycerophosphate loaded gelatin/sodium alginate injectable hydrogels for urinary incontinence treatment. Progress in Biomaterials, 10(2), 161-171.
[36] Mirbehbahani, F. S., Hejazi, F., Najmoddin, N., & Asefnejad, A. (2020). Artemisia annua L. as a promising medicinal plant for powerful wound healing applications. Progress in Biomaterials, 9(3), 139-151.
[37] Baillie, L.W., 1989. The effect of sodium alginate on the antibacterial activity of chlorhexidine, gentamicin and ciprofloxacin. The Journal of hospital infection, 14(2), pp.171-174.
[38] Ghahramanpoor, M.K., Najafabadi, S.A.H., Abdouss, M., Bagheri, F. and Eslaminejad, M.B., 2011. A hydrophobically-modified alginate gel system: utility in the repair of articular cartilage defects. Journal of Materials Science: Materials in Medicine, 22(10), pp.2365-2375.
[39] Tonks, L., 1967. The Birth of``Plasma''. American Journal of Physics, 35(9), pp.857-858.
[40] Huang, D., Hu, Z.D., Ding, Y., Zhen, Z.C., Lu, B., Ji, J.H. and Wang, G.X., 2019. Seawater degradable PVA/PCL blends with water-soluble polyvinyl alcohol as degradation accelerator. Polymer Degradation and Stability, 163, pp.195-205.
[41] Maghsoudlou, M. A., Barbaz Isfahani, R., Saber-Samandari, S., & Sadighi, M. (2021). The response of GFRP nanocomposites reinforced with functionalized SWCNT under low velocity impact: experimental and LS-DYNA simulation investigations. Iranian Journal of Materials Science and Engineering, 18(2), 0-0.
[42] Gitiara, Y., Barbaz-Isfahani, R., Saber-Samandari, S., & Sadighi, M. (2021). Effect of nanoparticles on the improving mechanical behavior of GFRP composites in a corrosive environment. Journal of Nanoanalysis.
[43] Barbaz-Isfahani, R., Saber-Samandari, S., & Salehi, M. (2022). Novel electrosprayed enhanced microcapsules with different nanoparticles containing healing agents in a single multicore microcapsule. International Journal of Biological Macromolecules, 200, 532-542.
[44] Yan, E., Cao, M., Wang, Y., Hao, X., Pei, S., Gao, J., Wang, Y., Zhang, Z. and Zhang, D., 2016. Gold nanorods contained polyvinyl alcohol/chitosan nanofiber matrix for cell imaging and drug delivery. Materials Science and Engineering: C, 58, pp.1090-1097.
[45] Kataria, K., Gupta, A., Rath, G., Mathur, R.B. and Dhakate, S.R., 2014. In vivo wound healing performance of drug loaded electrospun composite nanofibers transdermal patch. International journal of pharmaceutics, 469(1), pp.102-110.
[46] Karamian, E., Abdellahi, M., Khandan, A., & Abdellah, S. (2016). Introducing the fluorine doped natural hydroxyapatite-titania nanobiocomposite ceramic. Journal of Alloys and Compounds, 679, 375-383.
[47] Najafinezhad, A., Abdellahi, M., Ghayour, H., Soheily, A., Chami, A., & Khandan, A. (2017). A comparative study on the synthesis mechanism, bioactivity and mechanical properties of three silicate bioceramics. Materials Science and Engineering: C, 72, 259-267.
[48] Ghayour, H., Abdellahi, M., Ozada, N., Jabbrzare, S., & Khandan, A. (2017). Hyperthermia application of zinc doped nickel ferrite nanoparticles. Journal of Physics and Chemistry of Solids, 111, 464-472.
[49] Heydary, H. A., Karamian, E., Poorazizi, E., Khandan, A., & Heydaripour, J. (2015). A novel nano-fiber of Iranian gum tragacanth-polyvinyl alcohol/nanoclay composite for wound healing applications. Procedia Materials Science, 11, 176-182.
[50] Karamian, E., Khandan, A., Kalantar Motamedi, M. R., & Mirmohammadi, H. (2014). Surface characteristics and bioactivity of a novel natural HA/zircon nanocomposite coated on dental implants. BioMed research international, 2014.
[51] Jabbarzare, S., Abdellahi, M., Ghayour, H., Arpanahi, A., & Khandan, A. (2017). A study on the synthesis and magnetic properties of the cerium ferrite ceramic. Journal of Alloys and Compounds, 694, 800-807.
[52] Karamian, E. B., Motamedi, M. R., Mirmohammadi, K., Soltani, P. A., & Khandan, A. M. (2014). Correlation between crystallographic parameters and biodegradation rate of natural hydroxyapatite in physiological solutions. Indian J Sci Res, 4(3), 092-9.
[53] Khandan, A., & Esmaeili, S. (2019). Fabrication of polycaprolactone and polylactic acid shapeless scaffolds via fused deposition modelling technology. Journal of Advanced Materials and Processing, 7(4), 16-29.
[54] Fazlollahi, M., Morovvati, M. R., & Mollaei Dariani, B. (2019). Theoretical, numerical and experimental investigation of hydro-mechanical deep drawing of steel/polymer/steel sandwich sheets. Proceedings of the Institution of Mechanical Engineers, Part B: Journal of Engineering Manufacture, 233(5), 1529-1546.
[55] Morovvati, M. R., & Mollaei-Dariani, B. (2018). The formability investigation of CNT-reinforced aluminum nano-composite sheets manufactured by accumulative roll bonding. The International Journal of Advanced Manufacturing Technology, 95(9), 3523-3533.
[56] Kjaer, I. (2013). External root resorption: Different etiologies explained from the composition of the human root-close periodontal membrane. Dental Hypotheses, 4(3), 75.
[57] Motamedi, M. R. K., Behzadi, A., Khodadad, N., Zadeh, A. K., & Nilchian, F. (2014). Oral health and quality of life in children: a cross-sectional study. Dental Hypotheses, 5(2), 53.
[58] Narayanan, N., & Thangavelu, L. (2015). Salvia officinalis in dentistry. Dental Hypotheses, 6(1), 27.
[59] Khandan, A., Karamian, E., & Bonakdarchian, M. (2014). Mechanochemical synthesis evaluation of nanocrystalline bone-derived bioceramic powder using for bone tissue engineering. Dental Hypotheses, 5(4), 155.
[60] Shavarani, S. M., Nejad, M. G., Rismanchian, F., & Izbirak, G. (2018). Application of hierarchical facility location problem for optimization of a drone delivery system: a case study of Amazon prime air in the city of San Francisco. The International Journal of Advanced Manufacturing Technology, 95(9), 3141-3153.
[61] Mosallaeipour, S., Nejad, M. G., Shavarani, S. M., & Nazerian, R. (2018). Mobile robot scheduling for cycle time optimization in flow-shop cells, a case study. Production Engineering, 12(1), 83-94.
[62] Nejad, M. G., Güden, H., & Vizvári, B. (2019). Time minimization in flexible robotic cells considering intermediate input buffers: a comparative study of three well-known problems. International Journal of Computer Integrated Manufacturing, 32(8), 809-819.
[63] Ghasemi, M., Nejad, M. G., Alsaadi, N., Abdel-Jaber, M. T., Yajid, A., Shukri, M., & Habib, M. (2022). Performance measurment and lead-time reduction in epc project-based organizations: a mathematical modeling approach. Mathematical Problems in Engineering, 2022.
[64] Golabi, M., & Nejad, M. G. (2022). Intelligent and fuzzy UAV transportation applications in aviation 4.0. In Intelligent and Fuzzy Techniques in Aviation 4.0 (pp. 431-458). Springer, Cham.
[65] Nejad, M. G., & Kashan, A. H. (2019). An effective grouping evolution strategy algorithm enhanced with heuristic methods for assembly line balancing problem. Journal of Advanced Manufacturing Systems, 18(03), 487-509.
[66] Davani, P. P., Kloub, A. W. M., & Ghadiri Nejad, M. (2020). Optimizing the first type of U-shaped assembly line balancing problems. Annals of Optimization Theory and Practice, 3(4), 65-82.
[67] Chehrazi, M., & Moghadas, B. K. (2022). A review on CO2 capture with chilled ammonia and CO2 utilization in urea plant. Journal of CO2 Utilization, 61, 102030.
[68] Denizli, A., Ali, N., Bilal, M., Khan, A., & Nguyen, T. A. (Eds.). (2022). Nano-biosorbents for Decontamination of Water, Air, and Soil Pollution. Elsevier.
[69] Afrooz, M. R., Moghadas, B. K., & Tamjidi, S. (2022). Performance of functionalized bacterial as bio-adsorbent for intensifying heavy metal uptake from wastewater: A review study. Journal of Alloys and Compounds, 893, 162321.
[70] Moghadas, B. K., Esmaeili, H., Tamjidi, S., & Geramifard, A. (2022). Advantages of nanoadsorbents, biosorbents, and nanobiosorbents for contaminant removal. In Nano-Biosorbents for Decontamination of Water, Air, and Soil Pollution (pp. 105-133). Elsevier.
[71] Mirzapour, P., Kamyab Moghadas, B., Tamjidi, S., & Esmaeili, H. (2021). Activated carbon/bentonite/Fe3O4 nanocomposite for treatment of wastewater containing Reactive Red 198. Separation Science and Technology, 56(16), 2693-2707.
[72] Karimianmanesh, M., Azizifard, E., Javidanbashiz, N., Latifi, M., Ghorbani, A., Shahriari, S. (2021). Feasibility study of mechanical properties of alginates for neuroscience application using finite element method. Journal of Simulation and Analysis of Novel Technologies in Mechanical Engineering, 13(3), 53-62.