• Home
  • Biochemical, Molecular and Ecological characterization of Petroleum Biodegradable Bacteria in Misan Province/Iraq

Share To

Article Url


Manuscript ID : 686884 Visit : 209 Page: 115 - 129

10.22034/jchr.2021.686884

Article Type: Original Research

Biochemical, Molecular and Ecological characterization of Petroleum Biodegradable Bacteria in Misan Province/Iraq

Subject Areas : Journal of Chemical Health Risks

Hiba N. Dhaegheem 1 , Salih H. Jazza 2 , Zahid S. Aziz 3

1 - University of Misan, College of Sciences, Department of Biology, Misan, Iraq
2 - University of Misan, College of Sciences, Department of Biology, Misan, Iraq
3 - University of Misan, College of Sciences, Department of Biology, Misan, Iraq

Received: 2021-03-04 Accepted : 2021-10-25 Published : 2022-01-01

Keywords: biochemical, petroleum hydrocarbons, Biodegradation, Ecological, Molecular,

Abstract :

Oil-biodegradable bacterial strains were isolated, identified and characterized from oil contaminated soil samples from three oil refineries in Misan Province. Four bacterial isolates were characterized at biochemical levels by ordinary and standard bacteriological tests. The isolates were further identified by the Vitek-2 system. Three bacterial isolates (Sphingomonas paucimobilis, Novosphingobium subterraneum and Bacillus subtilis) were identified by universal primers and one bacterial isolate (Pseudomonas putida) by Pseudomonas sp. primer. The four bacterial isolates were grown on mineral salt media (MSM) supplemented with 0.5% crude oil and incubated at three incubation periods (7, 14 and 21) days were showed positive biodegradation of crude oil. The results revealed that the higher percentageof the degradation rate of n-alkanes by Pseudomonas putida (84.21%) followed by Bacillus subtilis (83.39%), Sphingomonas paucimobilis (79.63%) and Novosphingobium subterraneum (78.18%), while the higher percentage of the degradation rate of PAHs occurred by Sphingomonas paucimobilis (93.39%) followed by Novosphingobium subterraneum (92%), Pseudomonas putida (88. 84%) and Bacillus subtilis (85.99%) at the end of the incubation periods (21 days).

References:


  1. Abha S., Singh C.S., 2012. Hydrocarbon pollution: effects on living organisms, remediation of contaminated environments, and effects of heavy metals co-contamination on bioremediation. Introduction to enhanced oil recovery (EOR) processes and bioremediation of oil-contaminated sites. 185-206.
    2.
  2. Okoh E., Yelebe Z.R., Oruabena B., Nelson E.S., Indiamaowei O.P., 2020. Clean-up of crude oil-contaminated soils: bioremediation option. International Journal of Environmental Science and Technology. 17(2), 1185-1198.
    3.
  3. Hall D.O., Barnard G.W., Moss P.A., 2013. Biomass for energy in the developing countries: current role, potential, problems, prospects. Elsevier Science. 335p. doi:10.1016/C2013-0-03621-3.
    4.
  4. Chorom M., Sharifi H.S., Motamedi H., 2010. Bioremediation of a crude oil-polluted soil by application of fertilizers. Iran. J Environ Health Sci Eng. 7(4), 319-326.
    5.
  5. Fuentes S., Méndez V., Aguila P., Seeger M., 2014. Bioremediation of petroleum hydrocarbons: catabolic genes, microbial communities, and applications. Applied Microbiology and Biotechnology. 98(11), 4781-4794.
    6.
  6. Xu X., Lau W., Tain S., Wang W., Jiang P., Gao X., Li F., Li H., Yu H., 2018. Petroleum hydrocarbon-degrading bacteria for the remediation of oil pollution under aerobic conditions: a perspective analysis. Front Microbiol. 9. Doi: 10.3389/. fmicb .2018. 02885.
    7.
  7. Jiang Y., Brassington K.J., Prpich G., Paton G.I., Semple K.T., Pollard S.J.T., Coulon F., 2016. Insights into the biodegradation of weathered hydrocarbons in contaminated soils by bioaugmentation and nutrient stimulation. Chemosphere 161, 300–307.
    8.
  8. Akpe A.R., Ekundayo A.O., Esumeh F.I., 2013. Degradation of crude oil by bacteria: A role for plasmid-borne genes. Glob J Sci Front Res. 13(6), 20-6.
    9.
  9. Koukkou A., 2011. Microbial bioremediation of non-metals: Current Research. Caister Academic Press, UK.
    10.
  10. Ye S., Zeng G., Wu H., Zhang C., Dai J., Liang J., Zhang C., 2017. Biological technologies for the remediation of co-contaminated soil. Critical Reviews in Biotechnology. 37(8), 1062-1076.
    11.
  11. Kissel D.E., Sonon L., Vendrell P.F., Isaac R.A., 2009. Salt concentration and measurement of soil pH. Communications in Soil Science and Plant Analysis. 40(1-6), 179-187.
    12.
  12. Talal A.A., 2008. A study for the seasonal and regional variations of hydrocarbon levels and origin of n–alkanes in water, sediments and some species of Biota in Hor Al-Hammar Marshes. Ph.D thesis, college of science , University of Basrah . 166 p. [In Arabic]
    13.
  13. Goutex M., Saliot A., 1980. Relationship between dissolved and particulate fatty acid and hydrocarbons, Chlorophyll (a) and zooplankton biomass in Ville Franche Bay, Mediterranean Sea. Mar Chem. 8, 299-318.
    14.
  14. Saadoun I., 2002. Isolation and characterization of bacteria from crude petroleum oil contaminated soil and their potential to degrade diesel fuel. Journal of Basic Microbiology: An International Journal on Biochemistry, Physiology, Genetics, Morphology, and Ecology of Microorganisms. 42(6), 420-428.
    15.
  15. Khan K., Naeem M., Arshed M.J., Asif M., 2006. Extraction and characterization of oil degrading bacteria. J Ap Sc. 6(10), 2302-2306.
    16.
  16. Pincus D.H., 2006. Microbial identification using the bio Merieux Vitek®2 system Encyclopedia of Rapid Microbiological Methods.1-32.
    17.
  17. Dixit H., Lowry M., Mohsin U., Moond M., Kumar S., Chokriwal A., Gupta S., 2018. Screening and Identification of Diesel Oil Degrading Bacterial Isolates from Petroleum Contaminated soil of Barmer. J Pharm Chem Biol Sci. 6(1), 34-40.
    18.
  18. Xia M., Liu Y., Taylor A. A., Fu D., Khan A.R., Terry N., 2017. Crude oil depletion by bacterial strains isolated from a petroleum hydrocarbon impact solid waste management site in California. International Biodeterioration & Biodegradation 123, 70-77.      
    19.
  19. AL-Dee T.M., Malkawi H.I., 2009. Isolation, molecular and biochemical characterization of oil degrading bacteria from contaminated soil at an oil refinery Article. Journal of Applied Science and Technology (JAST). 14(1 & 2), 1 – 12.
    20.
  20. Obuekwe C.O., Al-Zarban S.S., 1998. Bioremediation of crude oil pollution in the Kuwaiti desert: the role of adherent microorganisms. Environment International. 24(8), 823-834.
    21.
  21. Al-Wasify R.S., Hamed S.R., 2014. Bacterial biodegradation of crude oil using local isolates. International Journal of Bacteriology. 3, 1-8. 10.1155/2014/863272.
    22.
  22. UNEP (United Nation Environment Program).,1989.Comparative toxicity test of water accommodated fraction of oils and oil dispersants to marine organisms. Reference methods for Marine pollution No. 45, 21.
    23.
  23. Al-Dossari M.A., 2008. Isolation and Identification of fungi from sediments of southern marshes of Iraq and study their ability to degrade crude oil in nitro. Ph.D. thesis, Basrah Univ. pp.113. [In Arabic].
    24.
  24. Gong Z., Li P., Wilke B. M., 2008. Effect of vegetable oil residue after soil extraction on physical-chemical properties of sandy soil and plant growth.Journal of Environmental Sciences. 20, 1458-1462. doi: 10.1016/S1001-0742(08)62549-8.
    25.
  25. Kisic I., Mesic S., Basic F., 2009.The effect of drilling fluids and crude oil on some chemical characteristics of soil and crops.Geoderma. 149(3-4), 209-216. 10.1016/j.geoderma. 2008.11.041.
    26.
  26. Barcenas-Moreno G., Gomes-Brandon M., Rousk J., Baath E., 2009. Adaptation of soil microbial communities to temperature: comparison of fungi and bacteria in a laboratory experiment. Glob Change Biol.15, 2950-2957.
    27.
  27. Fernandez-Calivino D., Baath E., 2010. Growth response of the bacterial community to ph in soils differing in ph. FEMS Microbiology Ecology. 73(1), 149-156.
    28.
  28. Wang L.C., Lin L.F., Lai S.O., 2009.Emissions of polycyclic aromatic hydrocarbons from fluidized and fixed bed incinerators disposing petrochemical industrial biological sludge. J Hazard Mater. 168, 438–444.
    29.
  29. Wang X.Y., Feng J., Zhao J.M., 2010. Effects of crude oil residuals on soil chemical properties in oil sites, Momoge Wetland, China. Environmental Monitoring and Assessment. 161(1), 271-280.
    30.
  30. Zhao B., Wang H., Mao X., Li R., 2009. A rapid screening method for bacteria degrading polycyclic aromatic hydrocarbons. Letters in Applied Microbiology. 49, 408-410.
    31.
  31. Obuekwe I.S., Semple K.T., 2013. Impact of Al and Fe on the development of phenanthrene catabolism in soil. Journal of Soils and Sediments. 13(9), 1589-1599.
    32.
  32. Hassanshahian M., EmtiaziI G., Cappello S., 2012. Isolation and characterization of crude-oil-degrading bacteria from the Persian Gulf and the Caspian Sea.Marine Pollution Bulletin. 64(1), 7-12.
    33.
  33. Mujahid T.Y., Wahab A., Padhiar S.H., Subhan S.A., Baloch M.N., Pirzada Z.A., 2015. Isolation and Characterization of Hydrocarbon Degrading Bacteria from Petrol Contaminated Soil. Journal of Basic & Applied Sciences.11, 223-231, 2015.
    34.
  34. Dash C., Payyappilli R.J., 2016. KOH string and Vancomycin susceptibility test as an alternative method to Gram staining. Journal of International Medicine and Dentistry. 3(2), 88-90.
    35.
  35. Lily M.K., Bahuguna A., Dangwal K., Garg V., 2009. Degradation of benzo(a)pyrene by a novel strain bacillus subtilis BMT4i (MTCC 9447). Brazilian Journal of Microbiology. 40, 884–892.
    36.
  36. Gupta B., 2012. Isolation and characterisation of Naphthalene degrading bacteria. A Master’s thesis submitted to the department of Environmental Science and Technology, Tharpar University, Patiala.
    37.
  37. Darsa K.V., Thatheyus J.A., Ramya D., 2014. Biodegradation of Petroleum compound using the bacterium Bacillus subtilis, Science International. 2(1), pp. 20–25.
    38.
  38. Sarang B., Richa K., Ram C., 2013. Comparative Study of Bioremediation of Hydrocarbon Fuel. Department of Biotechnology, Lovely Professional University, GT Road, Phagwara, Punjab, India. International Journal of Biotechnology and Bioengineering Research. 4(7), 677-686.
    39.
  39. Safiyanu I., AbdulwahidIsah A., Abubakar U.S., Rita Singh M., 2015. Review on Comparative Study on Bioremediation for Oil Spills Using Microbes. Research Journal of Pharmaceutical, Biological and Chemical Sciences. 6(6), 78
    40.
  40. Al-Taee A. M., Alkanany F.N., Gmais S.A., Alshawi H.A., 2017. Biodegradation of Aliphatic Hydrocarbon by Bacteria Isolated from Khor Al-Zubair Channel, Southern Iraq. International Journal of Marine Science. 7(18), 176-182.
    41.
  41. Barth H.J., 2003. The influence of cyanobacteria on oil polluted intertidal soils at the Saudi Arabian Gulf shores. Marine Pollution Bulletin. 46(10), 1245-1252.
    42.
  42. Sohn J.H., Kwon K.K., Kang J.H., Jung H.B., Kim S.J., 2004. Novosphingobium pentaromativorans sp. nov., a high-molecular mass polycyclic aromatic hydrocarbon-degrading bacterium isolated from estuarine sediment. Int J Syst Evol Microbiol. 54, 1483–1487.
    43.
  43. Liu Z.P., Wang B.J., Liu Y.H., Liu S.J., 2005. Novosphingobium taihuense sp. nov., a novel aromatic-compound-degrading bacterium isolated from Taihu Lake, China. Int J SystEvolMicrobiol. 55, 1229–1232.
    44.

44.Notomista E., Pennacchio F., CafaroV., Smaldone G., Izzo V., Troncone L., Di Donato A., 2011. The marine isolate Novosphingobium sp. PP1Y shows specific adaptation to use the aromatic fraction of fuels as the sole carbon and energy source. Microbial Ecology. 61(3), 582-594.         

  1. Mohammed A. K., Kadhim E.H., Jabbar N.M., 2017. Biodegradation of diesel contaminated soil using single bacterial strains and a mixed bacterial consortium. Al-Khwarizmi Engineering Journal. 13(4), 80-88.
    2.
  2. Jyothi K., Babu K.S., Clara N.K., Kashyap A., 2012. Identification and isolation of hydrocarbon degrading bacteria by molecular characterization. Helix. 2, 105-111.
    3.

Related articles

The rights to this website are owned by the Raimag Press Management System.
Copyright © 2021-2024

Home| Login| About Us| Contact Us|
[فارسی] [العربية] [fa] [ar]