Spectrofluorimetric Determination of Doxorubicin in Spiked Serum and Urine Samples

Subject Areas : Journal of Chemical Health Risks

Jamshid L. Manzoori ¹ , Jafar Abulhassani ² , Navid Niaei ³

1 - Department of Chemistry, Faculty of Science, Tabriz Branch, Islamic Azad University, Tabriz, Iran
2 - Department of Chemistry, Faculty of Science, Tabriz Branch, Islamic Azad University, Tabriz, Iran
3 - Department of Chemistry, Faculty of Science, Tabriz Branch, Islamic Azad University, Tabriz, Iran

Received: 2014-11-30 Accepted : 2018-10-29 Published : 2018-10-29

Keywords: Doxorubicin, Spectrofluorimetry, Terbium-sensitized, Deferasirox,

Abstract :

.A simple spectrofluorimetric method is described for the determination of doxorubicin (DXR) based on its quenching effect on the fluorescence intensity of Tb3+- deferasirox (DFX) complex as a fluorescent probe. The excitation and emission wavelengths were 328 and 545 nm, respectively. The effects of pH, time, order of addition of reagents, concentrations of Tb3+ and DFX and the buffer volume on the quenched fluorescence intensity were investigated and optimized. In the optimum conditions, the decrease of the fluorescence intensity of the system showed a good linear relationship with the concentration of DXR in the range of 20-1000 μg L-1, with a correlation coefficient 0.998. The detection limit (3s) was 6.1 μg L-1 and the relative standard deviation for four replicate determinations of different concentrations of DXR was in the range of 1.7–4.4%. The procedure was successfully applied to the determination of doxorubicin in urine and serum samples

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