ارزیابی لاین های اینبرد نوترکیب عدس با استفاده از شاخص های تحمل به خشکی
محورهای موضوعی : اکوفیزیولوژی گیاهان زراعیمحمد حسن رحیمی 1 , سعداله هوشمند 2 , محمود خدامباشی 3
1 - دانشگاه شهرکرد، دانشکده کشاورزی، گروه اصلاح نباتات و بیوتکنولوژی
2 - استاد گروه اصلاح نباتات و بیوتکنولوژی، دانشکده کشاورزی، دانشگاه شهرکرد، شهرکرد، ایران
3 - استاد گروه اصلاح نباتات و بیوتکنولوژی، دانشکده کشاورزی، دانشگاه شهرکرد، شهرکرد، ایران
کلید واژه: همبستگی, عملکرد دانه, شاخص های تحمل به خشکی, عدس, لاین اینبرد نوترکیب,
چکیده مقاله :
به‌منظور تحمل به خشکی 168 لاین اینبرد نوترکیب عدس حاصل از تلاقی رقم هندی L3685 (پر محصول، با عادت رشدی ایستاده و زودرس) به‌عنوان والد مادری و رقم ایرانی قزوین (با عادت رشدی خوابیده و دیررس) به‌عنوان والد پدری، آزمایشی تحت دو شرایط بدون تنش و تنش خشکی پایان دوره رشد در قالب طرح بلوک‌های کامل تصادفی با سه تکرار در مزرعه تحقیقاتی دانشگاه شهرکرد در سال زراعی 92-1391 به اجرا درآمد. نتایج تجزیه واریانس مرکب داده ها در شرایط فوق نشان داد که بین شرایط و همچنین بین لاین‌های مورد بررسی از نظر عملکرد دانه اختلاف معنی‌دار (P≤0.01) وجود دارند. معنی‌دار بودن اثر متقابل لاین × شرایط، بیانگر وجود اختلاف در لاین‌ها از نظر عملکرد دانه نسبت به تنش خشکی پایان دوره بود. لاین‌های 160، 125 و 129 در هر دو شرایط به‌عنوان متحمل ترین لاین‌ها به خشکی از لحاظ عملکرد دانه شناخته شدند. همبستگی مثبت و معنی‌دار بین شاخص‌های تحمل و عملکرد دانه در شرایط تنش و عدم تنش، بیـانگر برتـری شـاخص‌های STI، MP، HARM و GMP در شنـاسایی لاین‌های متحمل و حساس می باشند. بر اساس شاخص‌های فوق لاین‌های 160، 125، 48، 103 و 129 به‌عنوان لاین‌های متحمل شناخته شدند. با توجه به نتایج تجزیه به مؤلفه‌های اصلی، مؤلفه اول و دوم در مجموع 97/8 درصد از تنوع موجود در میان لاین‌ها برای شاخص‌های مقاومت به خشکی را توجیه نمودند. توزیع لاین‌ها در فضای بای پلات وجود تنوع ژنتیکی بین آنها نسبت به تنش خشکی را نشان داد و بر این اساس لاین‌های 160، 125، 48، 129 و 103 به‌عنوان لاین‌های متحمل و با عملکرد بالا شناسایی شدند.
To identify drought tolerant lines among 168 recombinant inbred lines derived from a cross between Hindi variety of L3685 (high yielding, erect type and early growth) as female parent and Iranian variety of Qazvin (with prostrate growth and late growth) as male parent, two experiments were conducted in randomized complete block design with three replications under non-stress and drought stress conditions at the experimental Field of Shahrekord University in 2013. Results of combined analysis of variance showed that there were significant differences among lines under study in both experimental conditions in terms of grain yield (P≤0.01). Significant interaction of line×conditions revealed that lines responded differently to drought stress based on grain yield. Results also indicates that lines 160, 125 and 129 were promising lines under both conditions in terms of grain yield. Positive and significant correlation between tolerance indices and grain yield under stress and non-stress conditions indicated that the STI, MP, HARM and GMP indices may identify promising from non promising lines. Based on the above criteria, lines 160, 125, 48, 103 and 129 were recognized as drought tolerant lines. According to the results of principal components analysis, the first and second components determined 97.8% of the total variations among genotypes under study for drought resistance indices. The distribution of lines in the biplot showed genetic variations to the drought stress and thus lines 160, 125, 48, 129 and 103 were identified as high yielders under drought stress condition.
Azizi Chakherchaman, Sh., H. Kezemi Arbat, M. Yarnia, and H. Mostafaei. 2009. Evaluation of drought tolerance in some improved lines and varieties of lentil (Lens culinaris L.) using stress suscebility and tolerance indices. Journal of Crop Production in Environmental Stress. 1(1): 1-12. (In Persian).
Babayeva, S., Z. Akparov, A. Damania, V. Izzatullayeva, G. Aslanova, and M. Abbasov. 2014. Genetic diversity for drought tolerance in lentils from central Asia and the Caucasus: CACLentil. Albanian Journal of Agricultural Science. 13(2): 1-8.
Bakhshayeshi, M., and M. Shekarchezade. 2015. Evaluation of genotypes of bread wheat (Triticum Aestivum) using drought tolerance indices. Journal of Crop Breeding. 7 (16): 49-59. (In Persian).
Bouslama, M., and W.T. Schapaugh. 1984. Stress tolerance in soybean. I: Evaluation of three screening techniques for heat and drought tolerance. Crop Science. 24: 933- 937.
Dhanda, S.S., G.S. Sethi, and R.K. Behl. 2004. Indices of drought tolerance in wheat genotypes at early stages of plant growth. Journal of Agronomy and Crop Science. 190: 6-12.
Erksine, W., A. Sarker, and S. Kumar. 2011. Crops that feed the world 3. Investing in lentil improvement toward a food secure world. Food Security. 3(2): 127-139.
FAO. 2013. http://faostat.fao.org/. Accessed November, 2013.
Farshadfar, E., M. Zamani, M. Motallebi, and A. Imamjomeh. 2001. Selection for drought resistance in chickpea lines. Iranian Journal of Agricultural Science. 32(1): 65-77. (In Persian).
Fernandez, G.C.J. 1992. Effective selection criteria for assessing plant stress tolerance. In Proceeding of the Symposium. Taiwan, 13-16 Aug. pp. 257-270.
Firuzi, B., A. Sofalian, M. Shekarpour, A. Rasulzadeh, and F. Ahmadpour. 2012. Evaluation of spring wheat genotypes using drought tolerance indices and principal components analysis. Environmental Stress in Crop Science. 5(2): 99-133. (In Persian).
Fisher, R.A., and R. Maurer. 1978. Drought resistance in spring wheat cultivars. I. grain yield response. Australian Journal of Agricultural Research. 29: 897-912.
Fouad, M., M. Imtiaz, S. Kumar, and R. Malhotra. 2011. Breeding food legumes for enhanced drought and heat tolerance to cope with climate change. In: Solh, M. and M.C. Saxena (Eds), Food Security and Climate Change in Dry Areas. Proceedings of International Conference. pp. 244–254.
Ganjali, A., A. Kafi, A. Bagheri, and F. Shahriyari. 2005. Screening for drought tolerance in chickpea genotypes (Cicer arietinum L.). Iranian Journal of Agricultural Sciences. 3 (1): 103-122 (In Persian).
Gavuzzi, P., F. Rizza, M. Palumbo, R.G. Campaline, G.L. Ricciardi, and B. Borghi. 1997. Evaluation of field and laboratory predictors of drought and heat tolerance in winter cereals. Canadian Journal of Plant Science. 77: 523- 531.
Golabadi, M., A. Arzani, and S.A.M. Maibody. 2006. Assessment of drought tolerance in segregating populations in durum wheat. African Journal of Agricultural Research. 5: 162–171.
Golparvar, A.R., A. Majidi Heravan, and A. Ghasemi Pir Bloti. 2004. Genetic improvement of yield potential and drought resistance in wheat genotypes (T. aestivum). Journal of Dryness and Drought Agricultural Extension. 3: 23-13.
Heidari Sharifabad, H. 2008. Drought mitigation strategies for the agriculture sector. The 10th Iranian Congress of Crop Science, 18-20 Aug. Karaj, Iran, pp. 47-62.
Jahani, J., Z. Yaghoobi Sagherchi, and A. Imani. 2014. Investigate the interaction of genotypes on the environment in terms of drought tolerance indices in the lentil genotypes. Journal of Applied Environmental and Biological Sciences. 4(3): 278-285.
Kristin, A.S., R.R. Senra, F.I. Perez, B.C. Enriques, J.A.A. Gallegos, P.R. Vallego, N. Wassimi, and J.D. Kelley. 1997. Improving common bean performance under drought stress. Crop Science. 37: 43-50.
Kumar, J., P.S. Basu, E. Srivastava, S.K. Chaturvedi, N. Nadarajan, and S. Kumar. 2012. Phenotyping of traits imparting drought tolerance in lentil. Crop and Pasture Science. 63(6): 547-554.
Moghaddaszadeh, M., M. Moghaddam Vahed, S. Aharizad, and S.A. Mohammadi. 2011. Evaluation of recombinant inbred lines of spring wheat under drought stress. Journal of Crop Ecophysiology. 6(1): 37-56. (In Persian).
Mohammad Alipour, H., M.R. Bihamta, S.A. Peighambari, and M.R. Naghavi. 2011. Evaluation of drought tolerance in Kabuli type chickpea genotypes. Seed and Plant Improvement Journal. 27(3): 393-409. (In Persian).
Mohammadi, R., M. Armion, D. Kahrizi, and A. Amri. 2010. Efficiency of screening techniques for evaluating durum wheat genotypes under mild drought conditions. International Journal of Plant Production. 4(1): 11-24.
Muscolo, A., A. Junker, C. Klukas, K. Weigelt-Fischer, D. Riewe, and T. Altmann. 2015. Phenotypic and metabolic responses to drought and salinity of four contrasting lentil accessions. Journal of Experimental Botany. 66 (18): 5467-5480.
Naroui Rad, M.R., A. Ghasemi, and A. Arjmandinejad. 2009. Study of limit irrigation on yield of lentil (Lens culinaris) genotypes of national plant gene bank of Iran by drought resistance indices. American-Eurasian Journal of Agricultural and Environmental Sciences. 6 (3): 352-355.
Neyestani, A., and M. Azimzadeh. 2003. Study of drought tolerance of 15 lentil varieties. Journal of Agricultural Science. 5(1): 61-69. (In Persian).
· Noroozi, M., and S.A.R. Kazemini. 2012. Effect of water stress and plant density on growth and seed yield of safflower. Iranian Journal of Field Crops Research. 10 (4): 781-788. (In Persian).Nourmand Moayyed, F., M.A. Rostami, and M.R. Ghanadha. 2001. Evaluate the indices of drought resistance in wheat. Iranian Journal of Agriculture Science. 32(4): 795-805. (In Persian).
Ouk, M., J. Basnayake, M. Tsubo, S. Fukai, K.S. Fischer, M. Cooper, and H. Nesbitt. 2006. Use of drought response index for identification of drought tolerant genotypes in rainfed lowland rice. Field Crops Research. 99: 48-58.
Pouresmael, M., M. Akbari, S. Vaezi, and S. Shahmoradi. 2009. Effects of drought stress gradient on agronomic traits in kabuli chickpea core collection. Iranian Journal of Crop Sciences. 11(4): 307-324 (In Persian).
· Pour Siahbidi, M.M., and A. Pour Aboughadareh. 2013. Evaluation of grain yield and repeatability of drought tolerance indices for screening chickpea (Cicer aritinum L.) genotypes under rainfed conditions. Iranian Journal of Genetics and Plant Breeding. 2: 28–37.Rosielle, A.A., and J. Hamblin. 1981. Theoretical aspects of selection for yield in stress and non-stress environment. Crop Science. 21: 943-946.
Salehi, M., A. Haghnazari, F. Shekari, and N. Nemati. 2005. The study of drought resistance indices in lentil (Lens Culinaris Medik). The 1st Iranaian Pulses Symposium. Mashhad, Iran, 20-21 November. pp. 230-234. (In Persian).
Seyfzadeh, S., J. Babaei Aghdam, A. Koohi- ghooshkhane, and M. Hanifi. 2014. Evaluation of seed yield and drought resistance indices in some lentil varieties. Journal of Crop Production Research. 6(1): 39-49. (In Persian).
Seyedzavar, J., M. Norouzi, and S. Aharizad. 2015. Evaluating tolerance indices of some new maize hybrids imposed to drought stress. Journal of Crop Ecophysiology. 9(4): 503-520.
Shafiei Khorshidi, M., M.R. Bihamta, F. Khialparast, and M.R. Naghavi. 2013. Comparison of some common bean (Phaseolus vulgaris L.) genotypes for drought tolerance. Iranian Journal of Field Crop Science. 44(1): 95-107. (In Persian).
Tadayyon, A., Hashemi, L. and M. Khodambashi. 2011. Effective morphological and phenological traits on seed and biological yield in lentil genotypes in Shahrekord region. Iranian Journal of Pulses Research. 2(2): 47-62. (In Persian).
Wang, W., B. Vinocur, and A. Altman. 2003. Plant responses to drought, salinity and extreme temperatures: towards genetic engineering for stress tolerance. Planta. 218: 1-14.
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Azizi Chakherchaman, Sh., H. Kezemi Arbat, M. Yarnia, and H. Mostafaei. 2009. Evaluation of drought tolerance in some improved lines and varieties of lentil (Lens culinaris L.) using stress suscebility and tolerance indices. Journal of Crop Production in Environmental Stress. 1(1): 1-12. (In Persian).
Babayeva, S., Z. Akparov, A. Damania, V. Izzatullayeva, G. Aslanova, and M. Abbasov. 2014. Genetic diversity for drought tolerance in lentils from central Asia and the Caucasus: CACLentil. Albanian Journal of Agricultural Science. 13(2): 1-8.
Bakhshayeshi, M., and M. Shekarchezade. 2015. Evaluation of genotypes of bread wheat (Triticum Aestivum) using drought tolerance indices. Journal of Crop Breeding. 7 (16): 49-59. (In Persian).
Bouslama, M., and W.T. Schapaugh. 1984. Stress tolerance in soybean. I: Evaluation of three screening techniques for heat and drought tolerance. Crop Science. 24: 933- 937.
Dhanda, S.S., G.S. Sethi, and R.K. Behl. 2004. Indices of drought tolerance in wheat genotypes at early stages of plant growth. Journal of Agronomy and Crop Science. 190: 6-12.
Erksine, W., A. Sarker, and S. Kumar. 2011. Crops that feed the world 3. Investing in lentil improvement toward a food secure world. Food Security. 3(2): 127-139.
FAO. 2013. http://faostat.fao.org/. Accessed November, 2013.
Farshadfar, E., M. Zamani, M. Motallebi, and A. Imamjomeh. 2001. Selection for drought resistance in chickpea lines. Iranian Journal of Agricultural Science. 32(1): 65-77. (In Persian).
Fernandez, G.C.J. 1992. Effective selection criteria for assessing plant stress tolerance. In Proceeding of the Symposium. Taiwan, 13-16 Aug. pp. 257-270.
Firuzi, B., A. Sofalian, M. Shekarpour, A. Rasulzadeh, and F. Ahmadpour. 2012. Evaluation of spring wheat genotypes using drought tolerance indices and principal components analysis. Environmental Stress in Crop Science. 5(2): 99-133. (In Persian).
Fisher, R.A., and R. Maurer. 1978. Drought resistance in spring wheat cultivars. I. grain yield response. Australian Journal of Agricultural Research. 29: 897-912.
Fouad, M., M. Imtiaz, S. Kumar, and R. Malhotra. 2011. Breeding food legumes for enhanced drought and heat tolerance to cope with climate change. In: Solh, M. and M.C. Saxena (Eds), Food Security and Climate Change in Dry Areas. Proceedings of International Conference. pp. 244–254.
Ganjali, A., A. Kafi, A. Bagheri, and F. Shahriyari. 2005. Screening for drought tolerance in chickpea genotypes (Cicer arietinum L.). Iranian Journal of Agricultural Sciences. 3 (1): 103-122 (In Persian).
Gavuzzi, P., F. Rizza, M. Palumbo, R.G. Campaline, G.L. Ricciardi, and B. Borghi. 1997. Evaluation of field and laboratory predictors of drought and heat tolerance in winter cereals. Canadian Journal of Plant Science. 77: 523- 531.
Golabadi, M., A. Arzani, and S.A.M. Maibody. 2006. Assessment of drought tolerance in segregating populations in durum wheat. African Journal of Agricultural Research. 5: 162–171.
Golparvar, A.R., A. Majidi Heravan, and A. Ghasemi Pir Bloti. 2004. Genetic improvement of yield potential and drought resistance in wheat genotypes (T. aestivum). Journal of Dryness and Drought Agricultural Extension. 3: 23-13.
Heidari Sharifabad, H. 2008. Drought mitigation strategies for the agriculture sector. The 10th Iranian Congress of Crop Science, 18-20 Aug. Karaj, Iran, pp. 47-62.
Jahani, J., Z. Yaghoobi Sagherchi, and A. Imani. 2014. Investigate the interaction of genotypes on the environment in terms of drought tolerance indices in the lentil genotypes. Journal of Applied Environmental and Biological Sciences. 4(3): 278-285.
Kristin, A.S., R.R. Senra, F.I. Perez, B.C. Enriques, J.A.A. Gallegos, P.R. Vallego, N. Wassimi, and J.D. Kelley. 1997. Improving common bean performance under drought stress. Crop Science. 37: 43-50.
Kumar, J., P.S. Basu, E. Srivastava, S.K. Chaturvedi, N. Nadarajan, and S. Kumar. 2012. Phenotyping of traits imparting drought tolerance in lentil. Crop and Pasture Science. 63(6): 547-554.
Moghaddaszadeh, M., M. Moghaddam Vahed, S. Aharizad, and S.A. Mohammadi. 2011. Evaluation of recombinant inbred lines of spring wheat under drought stress. Journal of Crop Ecophysiology. 6(1): 37-56. (In Persian).
Mohammad Alipour, H., M.R. Bihamta, S.A. Peighambari, and M.R. Naghavi. 2011. Evaluation of drought tolerance in Kabuli type chickpea genotypes. Seed and Plant Improvement Journal. 27(3): 393-409. (In Persian).
Mohammadi, R., M. Armion, D. Kahrizi, and A. Amri. 2010. Efficiency of screening techniques for evaluating durum wheat genotypes under mild drought conditions. International Journal of Plant Production. 4(1): 11-24.
Muscolo, A., A. Junker, C. Klukas, K. Weigelt-Fischer, D. Riewe, and T. Altmann. 2015. Phenotypic and metabolic responses to drought and salinity of four contrasting lentil accessions. Journal of Experimental Botany. 66 (18): 5467-5480.
Naroui Rad, M.R., A. Ghasemi, and A. Arjmandinejad. 2009. Study of limit irrigation on yield of lentil (Lens culinaris) genotypes of national plant gene bank of Iran by drought resistance indices. American-Eurasian Journal of Agricultural and Environmental Sciences. 6 (3): 352-355.
Neyestani, A., and M. Azimzadeh. 2003. Study of drought tolerance of 15 lentil varieties. Journal of Agricultural Science. 5(1): 61-69. (In Persian).
· Noroozi, M., and S.A.R. Kazemini. 2012. Effect of water stress and plant density on growth and seed yield of safflower. Iranian Journal of Field Crops Research. 10 (4): 781-788. (In Persian).Nourmand Moayyed, F., M.A. Rostami, and M.R. Ghanadha. 2001. Evaluate the indices of drought resistance in wheat. Iranian Journal of Agriculture Science. 32(4): 795-805. (In Persian).
Ouk, M., J. Basnayake, M. Tsubo, S. Fukai, K.S. Fischer, M. Cooper, and H. Nesbitt. 2006. Use of drought response index for identification of drought tolerant genotypes in rainfed lowland rice. Field Crops Research. 99: 48-58.
Pouresmael, M., M. Akbari, S. Vaezi, and S. Shahmoradi. 2009. Effects of drought stress gradient on agronomic traits in kabuli chickpea core collection. Iranian Journal of Crop Sciences. 11(4): 307-324 (In Persian).
· Pour Siahbidi, M.M., and A. Pour Aboughadareh. 2013. Evaluation of grain yield and repeatability of drought tolerance indices for screening chickpea (Cicer aritinum L.) genotypes under rainfed conditions. Iranian Journal of Genetics and Plant Breeding. 2: 28–37.Rosielle, A.A., and J. Hamblin. 1981. Theoretical aspects of selection for yield in stress and non-stress environment. Crop Science. 21: 943-946.
Salehi, M., A. Haghnazari, F. Shekari, and N. Nemati. 2005. The study of drought resistance indices in lentil (Lens Culinaris Medik). The 1st Iranaian Pulses Symposium. Mashhad, Iran, 20-21 November. pp. 230-234. (In Persian).
Seyfzadeh, S., J. Babaei Aghdam, A. Koohi- ghooshkhane, and M. Hanifi. 2014. Evaluation of seed yield and drought resistance indices in some lentil varieties. Journal of Crop Production Research. 6(1): 39-49. (In Persian).
Seyedzavar, J., M. Norouzi, and S. Aharizad. 2015. Evaluating tolerance indices of some new maize hybrids imposed to drought stress. Journal of Crop Ecophysiology. 9(4): 503-520.
Shafiei Khorshidi, M., M.R. Bihamta, F. Khialparast, and M.R. Naghavi. 2013. Comparison of some common bean (Phaseolus vulgaris L.) genotypes for drought tolerance. Iranian Journal of Field Crop Science. 44(1): 95-107. (In Persian).
Tadayyon, A., Hashemi, L. and M. Khodambashi. 2011. Effective morphological and phenological traits on seed and biological yield in lentil genotypes in Shahrekord region. Iranian Journal of Pulses Research. 2(2): 47-62. (In Persian).
Wang, W., B. Vinocur, and A. Altman. 2003. Plant responses to drought, salinity and extreme temperatures: towards genetic engineering for stress tolerance. Planta. 218: 1-14.
Zebarjadi, A., S. Tavakoli, A. Etminan, and R. Mohammadi. 2013. Evaluation of drought stress tolerance in durum wheat genotype using drought tolerance indices. Seed and Plant Improvement Journal. 29(1): 1-12. (In Persian).