Investigation of callus weight, total phenol, and total flavonoid contents in four different tissue types of Taxus baccata L.
Subject Areas : Plant Physiology
raheleh gorzi
1
,
Azizollah Kheiry
2
,
Mohsen Sanikhani
3
1 - the Department of Horticulture, Faculty of Agriculture University of Zanjan, IRAN
2 - عضو هیئت علمی دانشگاه زنجان
3 - Department of Horticultural Sciences, Faculty of Agriculture, University of Zanjan, Zanjan
Keywords: Taxus baccata, Taxol, callus weight, total phenol, total flavonoids,
Abstract :
Taxus baccata belongs to the Taxaceae family. Taxol is one of the most important natural anti-cancer compounds in the world, which is obtained from different taxus species. But due to the very small concentrations of this drug in the plant, suitable alternative methods should be used to produce this drug from the plant. Plant tissue callus and cell culture can be one of the most effective methods for producing the anticancer drug Taxol. In this research, callus formation and phenolic and flavonoid compounds of four different types of Taxus baccata L. tissues (stem, leaf, shoot tip and leaf + node) were investigated. For the induction of callus, four types of explants were placed in Gemburg culture medium with 6 mg/L 2,4-D, 0.5 mg/L kinetin and 30 g/L sucrose. After 4 weeks, the fresh weight and dry weight of callus, total phenols, and total flavonoids were measured in four types of tissue. The results showed that the highest fresh and dry weight of callus belonged to the shoot tip tissue while the lowest fresh and dry weight of callus belonged to the leaf tissue. Also, the total phenol and flavonoid contents of the four tissues under study showed that the lowest values of total phenol and total flavonoid belonged to the shoot tip tissue and other tissues were not different from each other.
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