In vitro micro propagation, callus induction and shoot regeneration in safflower L. cv. Lesaf. Hamidreza Ghasempour1,3*, Zhaleh Soheilikhah1 ,Ali Reza Zebarjadi2, Saba Ghasempour4 and Naser Karimi3
Subject Areas : Phytochemistry
Keywords: NAA, Safflower, shoot regeneration, Callus induction, Kinetin, 2, 4-D, BAP,
Abstract :
The present study was conducted to induce callus from different in vitro grown seedling explants of shoot, root and leaf segments in order to develop shoot buds and rooting from callus. The sterilized seeds were cultured on Murashige and Skoog medium, and then the explants were cultured from seedling and transferred to an MS medium supplemented with different concentrations of BAP, kinetin, NAA, and 2, 4-D growth regulator hormones. Among all explants, the leaf explants should produce significant callus induction (97.79 %) on MS + 1.0 mgl-1 BAP medium. After 4 weeks, the obtained calluses were transferred to a new regeneration media with different concentrations of BAP and NAA and then the calluses were sub cultured on the same medium every 2 weeks. Overall, the maximum regeneration frequency was obtained from MS medium containing 0.1 mgl-1 NAA and 1.0 mgl-1 BAP.