Investigation of (Stearoyl-CoA Desaturase 1) SCD1 Gene Polymorphism in Khuzestan Buffalo Population Using PCR-RFLPMethod

Subject Areas : Camel

ک. تقی‌زاده ¹ , م.ت. بیگی نصیری ² , ج. فیاضی ³ , م. بوجارپور ⁴

1 - Department of Animal Science, Ramin Agricultural and Natural Resources University, Mollasani, Ahvaz, Iran
2 - Department of Animal Science, Ramin Agricultural and Natural Resources University, Mollasani, Ahvaz, Iran
3 - Department of Animal Science, Ramin Agricultural and Natural Resources University, Mollasani, Ahvaz, Iran
4 - Department of Animal Science, Ramin Agricultural and Natural Resources University, Mollasani, Ahvaz, Iran

Received: 2013-06-14 Accepted : 2013-08-01 Published : 2014-06-01

Keywords: PCR-RFLP, Buffalo, Hardy-Weinberg equilibrium, MUFA, SCD1 gene,

Abstract :

Stearoyl-CoA desaturase (SCD) is a rate-limiting enzyme in the biosynthesis of monounsaturated fatty acids (MUFA). A number of studies support the hypothesis that SCD gene regulation and polymorphism may affect fatty acid composition and fat quality in meat and milk. Single nucleotide polymorphisms in the coding region of the bovine stearoyl-CoA desaturase gene have been predicted to result in p. 293A (alanine at amino acid 293) and p. 293V (valine at amino acid 293) alleles at the stearoyl-CoA desaturase1 locus. The objective of this study was to characterize polymorphisms of stearoyl-CoA desaturase 1 (SCD1) gene in Khuzestan buffalo population. This is the first study of SCD1 gene polymorphism in Iranian buffalo. The blood samples were taken from 85 buffalo of Khuzestan-Iran population. Genomic DNA was extracted using a kit DIAtom DNA prep. After this, PCR reactions were made by using primers that encloses exon V. A 400 bp fragment amplified with standard PCR method. The PCR products were digested by NCO1 restriction enzyme with PCR-RFLP method. All samples were genotyped on 3% agarose gels stained with ethidium bromide. Electrophoretic mobility shift determined that there were not genetic differences between these animals in the studied region by using NCOI enzyme. Also, this study showed that genotype frequencies were not in Hardy-Weinberg equilibrium for SCD1 gene.

References:

Arnould V.M.R. and H. Soyeurt (2009). Genetic variability of milk fatty acids. J. Appl. Genet. 50, 29-39.
Camargo G.M.F., Thomazin R.B., Boarini F.G. and Tonhati H. (2007). Use of PCR-RFLP (polymerase chain reaction-restricted fragment length polymorphism) in the gene of the enzyme Stearoyl-CoA-desaturase in Bubalus bubalis. J. Anim. Sci. 6, 307-309.
Campbell E.M.G., Gallagher D.S, Davis K.S., Taylor J.F. and Smith S.B. (2001). Rapid communication: mapping of the bovine stearoyl coenzyme A desaturase (SCD) gene to BTA26. J. Anim. Sci. 79, 1954-1955.
Conte G.M., Mele S., Chessa B., Castiglioni A., Serra G., Pagnacco G. and Secchiari P. (2010). Diacylglycerol acyltransferase 1, stearoyl-CoA desaturase 1, and sterol regulatory element binding protein 1 gene polymorphisms and milk fatty acid composition in Italian Brown cattle. J. Dairy Sci. 93, 753-763.
Dobrzyna A. and Ntambi J.M. (2005). The role of stearoyl-CoA desaturase in the control of metabolism. Prost. Leuk. Essent. Fatty Acids. 73, 35-41.
Grummer R.R. (1991). Effect of feed on the composition of milk fat. J. Dairy Sci. 74, 3244-3257.
Hu F.B., Manson J.E. and Willet W.C. (2001). Types of dietary fat and risk of coronary heart disease: a critical review. J. Am. Coll. Nutr. 20, 5-19.
Kgwatalala P.M., Ibeagha-Awemu E.M., Hayes J.F. and Zhao X. (2007). Single nucleotide polymorphisms in the open reading frame of the stearoyl-CoA deasturase gene and the resulting genetic variants in Canadian Holstein and Jersey cows. DNA Sequence. 18, 357-362.
Kgwatalala P.M., Ibeagha-Awemu E.M., Mustafa A.F. and Zhao X. (2009). Influence of stearoyl-coenzyme A desaturase 1 genotype and stage of lactation on fatty acid composition of Canadian Jersey cows. J. Dairy Sci. 92, 1220-1228.
Keating A.F., Kennelly J.J. and Zhao F. (2006). Characterization and regulation of the bovine stearoyl-CoA desaturase gene promoter. Biochem. Biophys. Res. Commun. 344, 233-240.
Mainieri D., Summermatter S., Seydoux J., Montani J.P., Rusconi S., Russell A.P., Boss O., Buchala A.J. and Dulloo A.G. (2006). A role for skeletal muscle stearoyl-CoA desaturase 1 in control of thermogenesis. Fed. Am. Soc. Exp. Biol. J. 20(10), 1157-1170.
Mauvoisin D. and Mounier C. (2011). Hormonal and nutritional regulation of SCD1 gene expression. Biochimie. 93, 78-86.
Moioli B., Contarini G., Avalli A., Catillo G., Orru L., De Matteis G., Masoero G. and Napolitano F. (2007). Short communication: effect of stearoyl-Coenzyme A desaturase polymorphisms on fatty acid composition of milk. J. Dairy Sci. 90, 3553-3558.
Moore J.H. and Christie W.W. (1979). Lipid metabolism in the mammary gland of ruminant animal. Prog. Lipid Res. 17, 347-395.
Ntambi J.M. and Miyazaki M. (2004). Regulation of stearoyl-CoA desaturases and role in metabolism, Prog. Lipid Res. 43, 91-104.
Ntambi J.M., Miyazaki M., Stoehr J.P., Lan H., Kendziorski C.M., Yandell B.S., Song Y., Cohen P., Friedman J.M. and Attie A.D. (2002). Loss of stearoyl-CoA desaturase 1 function protects mice against adiposity. Proc. Natl. Acad. Sci. 99(17), 11482-11486.
Schennink A., Heck J.M.L., Bovenhuis H., Visker M.H.P.W., van Valenberg H.J.F. and van Arendonk J.A.M. (2008). Milk fatty acid unsaturation: genetic parameters and effects of stearoyl-CoA desaturase 1 (SCD1) and acyl-CoA: diacylglycerol acyltransferase 1 (DGAT1). J. Dairy Sci. 91, 2135-2143.
Taniguchi M., Utsugi T., Oyama K., Manna H., Kobayashi M., Tanab Y., Ogino A. and Tsuji S. (2004). Genotype of stearoyl-CoA desaturase is associated with fatty acid composition in Japanese Black cattle. J. Mamm. Gen. 14, 142-148.