Timing Ovulation in Ewes Treated with Ovsynch Different Times of PGF2 αInjection during the Breeding Season
Subject Areas : Camel
1 - Animal Production Research Institute, Ministry of Agriculture, Egypt
Keywords: litter size, progesterone, Estrus, ewes, lambing, Ovsynch,
Abstract :
This study was carried out at Sakha Animal Production Research Station, during the period from Oct., 2009 to Sept., 2010. Forty Rahmani ewes were divided into three treatment groups: the 1st, 2nd and 3rd treatment groups were intramuscularly injected (Day 0) with 1 mL GnRH analogue followed by an intramuscular injection with 0.7 mL PGF2α5 (G1), 6 (G2) or 7 (G3) days later. A second dose of 1 mL GnRH analogue was given on day 7 (G1), 8 (G2) or 9 (G3), and artificially insemination of treated doze was carried out 24 h later, while the 4th group represented the control ewes which were allowed for natural mating from 1st to the end of January, the breeding season. Results show that one out of 10 ewes (10%) exhibited estrous activity in G1 versus 30% (3 out of 10 ewes) in both G2 and G3. Ewes in G1 treatment group showed highest (P<0.05) lambing rate (60%), followed by G2 (50%) and the lowest in G3 (40%), while, lambing rate of the controls was 60%. Litter size and fecundity were significantly the highest (P<0.05) in G1 (1.67/litter and 100.2%), followed by G2 (1.40/litter and 70%) and the lowest in G3 (1.25/litter and 43%), respectively. P4 concentration was significantly (P<0.05) increased in all treatment groups as affected by the 1st GnRH injection, thereafter, it showed marked decrease in all treatment groups post-PGF2ainjection. Post-2nd GnRH injection, P4 level showed again a pronounced increase in all treatment groups. On day 21-24 post-mating, P4 level showed the highest values in all treatment groups. Based on the foregoing results, using GnRH-PGF2-GnRH (GnRH, 0 d; PGF2α 5 d laterandGnRH 48 h later) protocol during breeding season can be used for synchronization of estrus and ovulation to reduce service and lambing interval of ewes in the large flocks.
Alaçam E. (1994). Üremenin kontrolü, In ‘Evcil Hayvanlarda Doğum Ve İnfertilite’ Edited By E. Alaçam (Medisan-Ankara), pp. 71-77.
Ataman M.B. and Aköz M. (2006). GnRH-PGF2α and PGF2α-
PGF2α synchronization in Akkaraman cross-breed sheep in the breeding season. Bull. Vet. Inst. Pulawy. 50, 101-104.
Bane A.A. (1982). Study on the technique of hemocytometric determination of sperm motility and sperm concentration in bull semen. Cornell Vet. 42, 518-528.
Barid D.T. (1978). Pulsatile secretion of LH and ovarian estradiol during the follicular phase of the sheep estrous cycle. Biol. Reprod. 18, 359-364.
Barid D.F. and Scaramuzzi R.J. (1976). Changes in the secretion of ovarian steroids and pituitary LH in the pre-ovulatory period in the ewe: the effect of progesterone. J. Endocrinol. 70, 237-245.
Bartlewski P.M., Beard A.P. and RawlingsN.C.(2000). Ultrasonographic study of ovarian function during early pregnancy and after parturition in the ewe. Theriogenology. 53, 673-689.
Beck N.F.G., Peters A.R. and Williams S.P. (1994). The effect of GnRH agonist (Buserelin) treatment on day 12 post mating on the reproductive performance of ewes. Anim. Prod. 48, 243-247.
Beck N.F.G., Jones M., Davies B., Peters A.R. and Williams S.P. (1996). Oestrus synchronization in ewes: the effect of combining a prostaglandin analogue with a GnRH agonist (buserelin). Anim. Sci. 62, 85-87.
Bojanic S., LakeR., Place J., Jones L., Laycoock J., Carter G. and Alagh-Band-Zadeh J. (1991). Serum progesterone concentration is raised during early follicular phase in women with polycystic ovaries. Ann. Clin. Biochem. 28, 105-106.
Braden T.D., Sawyer H.R. and Niswender G.D. (1989). Functional and morphological characteristics of the first corpus luteum formed after parturition in ewes. J. Reprod. Fertil. 86, 523-533.
Cahill L.P. (1981). Folliculaogenesis in the sheep as influenced by breed, season and oestrous cycle. J. Reprod. Fertil. (Suppl) 30, 135-142.
Dancan D.B. (1955). T-test and interval for comparison suggested by the date. Biometrics. 31, 339-359.
El-Gohary E.S. (2006). Physiological studies on sheep. Ph.D. Thesis, Fac. Agric., Mansoura Univ., Egypt.
Fitzgerald J.A., Ruggles A.J., Stellflug J.N. and Hansel W. (1985). A seven-day synchronization method for ewes using medroxyprogesterone acetate (MAP) and prostaglandin F2α. J. Anim. Sci. 61, 466-469.
Ginther O.J., Wiltbank M.C., Fricck M.M., Gibbons J.R. and Kot K. (1996). Selection of the dominant follicle in cattle. Biol. Reprod. 55, 1187-1194.
Goodman B.L. and Karsch F.J. (1980). Pulsatile secretion of luteinizaing hormone: differential suppression by ovarian steroids. Endocrinology. 107, 1286-1290.
Goodman B.L., Reichert L.E., Legan S.J., Ryan K.D., Foster D.L. and Karsch F.J. (1981). Role of gonadotropins and progesterone in determining the preovulatory rise in the ewe. Biol. Reprod. 25, 134-142.
Horoz H., Ak K., Kilicarslan R. and Sönmez C. (1999). Üreme mevsimindeki Sakız koyunlarında PGF2α ile östrüs senkronizasyonunun serum progesterone, östradiöl 17-β ve LH üzerine etkisi. IU Vet. Fak. Derg. 25, 89-96.
Husein M.Q. and Kridli R.T. (2002). Reproductive responses of Awassi ewes treated with either naturally occurring progesterone or synthesis progestagen. Asian-Aust. J. Anim. Sci. 15, 1257-1262.
Husein M.Q. and Kridli R.T. (2003). Effect of progesterone prior to GnRH-PGF2α treatment on induction of oestrus and pregnancy in anoestrus Awassi ewes. Reprod. Domest. Anim. 38, 228-232.
Karsch F.J. and Foster D.L. (1981). Environmental control of seasonal breeding a common final mechanism governing seasonal breeding and sexual maturation. In: Environmental Factors Mammalian Reproduction, Eds. D. P. Gilmore and B. Cooke. Macmillan Press Ltd., London.
Karsch F.J., Foster D.L., Tegan S.L., Ryan K.D. and Peter G.K. (1979). Control of the preovulatory endocrine evenets in the ewe: Interrelationship of estradiol, progesterone and luteinizing hormone. Endocrinology. 105, 421-426.
Lamb G.C. (2002). Review of estrous synchronization systems: GnRH. Proceedings: The Applied Reproductive Strategies Beef Cattle Workshop, p. 23-32, Manhattan, KS.
Larsson B., Gustafsson A., Nasholm A. and Bjurström L. (1991). A programme for oestrus synchronization and embryo transfer in sheep. Reprod. Domest. Anim. 26, 301-308.
Leboeuf B., Restall B. and Salamon S. (2000). Production and storage of goat semen for artificial insemination. Anim. Reprod. Sci. 62, 131-141.
Levine J.E., PauK.Y., Ramirez V.D. and Jackson G.L. (1982). Simultaneous measurement of luteinizing hormone-releasing hormone and luteinizing hormone release in unanesthetized, ovariectomized sheep. Endocrinology. 111, 1449-55.
Lussier J.G., Matton P., Guilbault L.A., Grasso F., Mapletoft R.J. and Carruthers T.D. (1987). Ovarian follicular development and endocrine responses in follicular-fluidtreated and hemiovariectomized heifers. J. Reprod. Fertil. 102, 95-105.
Mihm M. and Bleach E.C.L. (2003). Endocrine regulation of ovarian follicle development in cattle. Anim Reprod Sci. 78, 217–237.
Mihm M., Curran N., Hyttel P., Knight P.G., Boland M.P. and Roche J.F. (1999). Effect of dominant follicle persistence on follicular fluid oestradiol and inhibin and on oocyte maturation in heifers. J. Reprod. Fertil. 116, 293-304.
Nephew K.P., Cardenas H., McClure K.E., Ott T.E., Bazer F.W. and Pope W.F. (1994). Effects of administration of human chorionic gonadotropin or progesterone before maternal recognition of pregnancy on blastocyst development and pregnancy in sheep. J. Anim. Sci. 72, 453-458.
Örsan G., Pancarci S.M., Çenesiz M., Beceriklisoy H.B. and Kanca H. (2007). Effect of GnRH implant (deslorelin) with different formulation on fertility and delay of oestrous cycle in ewes. Bull. Vet. Inst. Pulawy.51, 25-29.
Öztürkler Y., Baykal A. and Ven B.G. (2003). Combined effect of a prostaglandin analogue and a progestagen treatment for 5 days on oestrus synchronisation in Tushin ewes. Indian Vet. J. 80, 917-920.
Pursley J.R., Silcox R.W. and Wiltbank M.C. (1998). Effect of time of artificial insemination on pregnancy rates, calving rates, pregnancy loss, and gender ratio after synchronization of ovulation in lactating dairy cows. J. Dairy Sci. 81, 2139-2144.
RawlingsN.C.and Cook S.J. (1993). LH secretion around the time of the preovulatory gonadotrophin surge in the ewe. Anim. Reprod. Sci. 30, 289-299.
Roberts R.M., Godkin J.D., Bazer F.W., Fincher K.B., Thatcher W.W., Knickerbocker J. and Bartol F.F. (1985). Antiluteolysins produced by mammalian conceptuses. In: Edwards K.G., Purdy J., Steptoe P.J. (Eds.), Implantation of the Human Embryo. London: Academic Press, pp. 253-282.
SAS/STAT. (2000). Guide for personal computers. Version 8 Ed. Cary NC, USA, SAS Institute.
Schirar A., Meusnier C., PalyJ., Lavesseur M.C. and Martinet J. (1989). Resumption of ovarian activity in postpartum ewes: role of the uterus. Anim. Reprod. Sci. 19, 79-91.
Simonetti L., M. B. Blanco M.R. and Gardon J.C. (2000). Estrus synchronization in ewes treated with sponges impr egnated with different doses of medroxyprogesterone acetate. Small Ruminant Res. 38, 243-247.
Snedecor G.W. and Cochran W.G. (1982). Statistical Methods, 7th Ed., IowaState University.
Stevenson J.S., Kobatashi Y., Shipka M.P. and Raucholz K.C. (1996). Altering conception of dairy cattle by gonadotropin-releasing hormone preceding luteolysis induced by prostaglandin F2α. J. Dairy. Sci. 79, 402-410.
Sunderland S.J., Crowe M.A., Boland M.P., Roche J.F. and IrelandJ.J. (1994). Selection, dominance and atresia of follicles during the oestrous cycle of heifers. J. Reprod. Fertil. 101, 547-555.
Twagiramungu H., Guilbault L.A., Proulx J. and Dufour J.J. (1992). Synchronization of estrus and fertility of beef cattle with two injections of Buserelin and prostaglandin. Theriogenology. 38, 1131-114.
Wallace J.M., Ashworth C.J., Aitken R.P. and Cheyne M.A. (1992). Corpus luteum function in ewes postpartum: a study in vivo and in vitro. Reprod. Fertil. Devel. 4, 77-90.
Wildeus S. (1999). Current concepts in synchronization of estrus: Sheep and goats. Proc. Am. Soc. Anim. Sci. Available: http://www.asas.org/JAS/symposia/proceedings/0016.pdf.