Investigation of the CYP2C9 Gene polymorphism frequency and its effects on the warfarin dose used by the patients in a population from Iran

Subject Areas :

Farideh Olia ¹ , Morteza Seifati ² , Naghmeh Satarzadeh ³ , Shahriar Dabiri ⁴ , Shima Mirzaie parsa ⁵ , Mohammad Javad Soltani-Banavand ^{6 *}

1 - Department of Biology, Ashk.C., Islamic Azad University, Ashkzare, Iran
2 - Department of Biology, Ashk.C., Islamic Azad University, Ashkzare, Iran
3 - Department of Pharmaceutical Biotechnology, Faculty of Pharmacy, Kerman University of Medical Sciences, Kerman, Iran
4 - Pathology Department, Afzalipour School of Medicine, Kerman University of Medical Sciences, Kerman, Iran
5 - Department of Biology, Da.C., Islamic Azad University, Damghan, Iran
6 - Faculty of Medicine, Ke.C., Islamic Azad University, Kerman, Iran

Received: 2024-11-23 Accepted : 2025-08-02 Published : 2025-09-13

Keywords: Warfarin, PCR-RFLP, Gene Polymorphism, CYP2C9, Vitamin k ,

Abstract :

Abstract Background & Aims: warfarin is an edible and anticoagulant drug that is used to treat and avoid thrombolytic disorders. Despite its efficacy, this substance has a therapeutic index and the response to it is completely variable. plenty of this drug lead to bleeding and low dose of that lead to poor blood coagulation. The aim of this study was to survey the relationship between dose of usage of warfarin and Gene polymorphism and its relation with age and sex of the patient. Methods: Blood sampling was done intravenously by receiving consent form on a total of 100 people with heart disease who were referred to a medical laboratory in the city of Kerman to perform the PT Test. After DNA extraction the PCR_RFLP method was done on all samples. the frequency of CYP2C9 gene polymorphism and the relationship with the dose of usage of warfarin, age and gender of patients has been investigated. Results: The frequency of observerd genotypes were 1%, 12%, 87%, sequentially CC, TC, TT In the present study. There was meaningful relationship between age and the dose of usage of warfarin (P=0.0001). Data analysis showed that there was no meaningful relationship between variant genotypes CYP2C9 and the dose of usage of warfarin (P=0.999) and also male and female groups in terms of relationship genotype polymorphism (P=0.115). Conclusions: Based on the results it seems that other genes interfere in the usage of warfarin according to genotype of each population.