In vivo assessment of probioticated African Yam Bean (Sphenostylis stenocarpa)-based milk analogue

Subject Areas :

Esther Adurotoye ¹ , Abiodun Victor Ikujenlola ² , Hezekiah Adeniran ³

1 - Department of Food Science and Technology, Obafemi Awolowo University, Ile-Ife, Nigeria
2 - Department of Food Science and Technology, Obafemi Awolowo University, Ile-Ife, Nigeria
3 - Department of Food Science and Technology, Obafemi Awolowo University, Ile-Ife, Nigeria

Received: 2020-07-01 Accepted : 2020-09-15 Published : 2020-09-01

Keywords: Histopathological, aspartate transaminase, <i>Escherichia coli</i>, Probiotic, milk analogues,

Abstract :

This study investigated the in vivo effect of administration of probioticated African Yam Bean (AYB) based milk analogues on albino rats. Vegetable milk extracts were obtained from processed African Yam Bean, Soybean Seeds, and Coconut. The samples were mixed at different ratios of 1:1:1, 3:1:1, and 5:1:1 (African Yam Bean: Soybean: Coconut) as A, B, and C, respectively. The blended milk analogues were fermented using Lactobacillus delbrueckii isolated from Kununzaki drink. The effect of the fermented milk analogues on the intestinal tract and the serum of the albino rats was also investigated. This strain inhibited the growth of Escherichia coli, a selected food-borne pathogen in vivo. Animals fed with only E. coli had the highest AST and ALT values of 79.31 and 24.59 IU/L respectively. Animals fed with sample B1 had the lowest ALT value of 16.24 IU/L. The weight gain was highest in animals fed with only probiotic drink sample. The histopathological examination showed the protective effect of the group dosed with the probiotic drink alone and the ones fed with a higher proportion of AYB. The study concluded that probioticated African yam bean drink exhibited health-promoting effect in vivo on the experimental animals and hence could be used as probiotic drink.

References:

1. Oyetayo V, Adetuyi F, Akinyosoye F. Safety and protective effect of Lactobacillus acidophilus and Lactobacillus casei used as probiotic agent in vivo. African Journal of Biotechnology. 2003;2(11):448-52.


2. Soccol CR, Vandenberghe LPdS, Spier MR, Medeiros ABP, Yamaguishi CT, Lindner JDD, et al. The potential of probiotics: a review. Food Technology and Biotechnology. 2010;48(4):413-34.


3. Casas IA, Dobrogosz WJ. Validation of the probiotic concept: Lactobacillus reuteri confers broad-spectrum protection against disease in humans and animals. Microbial Ecology in Health and Disease. 2000;12(4):247-85.


4. Bertazzoni M, Benini A, Marzotto M, Hendriks H, Sbarbati A, Dellaglio F, editors. Preliminary screening of health-promoting properties of new lactobacillus strain: in vitro and in vivo. HEALFO abstracts, Italy; 2001.


5. Chang CY, Stone MB. Effect of total soymilk solids on acid production by selected Lactobacilli. Journal of Food Science. 1990;55(6):1643-6.


6. Silva A, Bambirra E, Oliveira A, Souza P, Gomes D, Vieira E, et al. Protective effect of bifidus milk on the experimental infection with Salmonella enteritidis subsp. typhimurium in conventional and gnotobiotic mice. Journal of Applied Microbiology. 1999;86(2):331-6.


7. Yilmaz-Ersan L, Kurdal E. The production of set-type-bio-yoghurt with commercial probiotic culture. International Journal of Chemical Engineering and Applications. 2014;5(5):402.


8. Adeniran H, Olaniyi O, Abiose S. Viability and effect of' probiotic' lactic acid bacteria isolated from locally fermented foods in a non-dairy beverage from African Yam Bean. Ife Journal of Technology. 2016;24(1):11-7.


9. Osundahunsi O, Amosu D, Ifesan B. Quality evaluation and acceptability of soy-yoghurt with different. American Journal of Food Technology. 2007;2(4):273-80.


10. Ebhodaghe S, Abiose S, Adeniran H. Assessment of physico-chemical characteristics, viability and inhibitory effect of Bifidobacteria in soymilk. Journal of Food Research. 2012;1(2):159.


11. Ikujenlola AV, Adurotoye EA, Adeniran HA. Chemical and sensory properties of probioticated drinks from blends of African yam bean, soybean and coconut milk analogues. Acta Universitatis Cibiniensis Series E: Food Technology. 2019;23(2):147-56.


12. Kasarala G, Tillmann HL. Standard liver tests. Clinical Liver Disease. 2016;8(1):13.


13. Hasan KMM, Tamanna N, Haque MA. Biochemical and histopathological profiling of Wistar rat treated with Brassica napus as a supplementary feed. Food Science and Human Wellness. 2018;7(1):77-82.


14. Botros M, Sikaris KA. The de ritis ratio: the test of time. The Clinical Biochemist Reviews. 2013;34(3):117.


15. Aminigo E, Lehtola P, Metzger L. Nutritive composition and physical characteristics of supplemented limitation milk from African yam bean (Sphenostylis stenocarpa). Global Journal of Pure and Applied Sciences. 2007;13(4):457-62.


16. Udeozor LO. Tigernut-soy milk drink: preparation, proximate composition and sensory qualities. International Journal of Food and Nutrition Science. 2012;1(4):18-26.


17. Sanful RE. The use of tiger-nut (Cyperus esculentus), cow milk and their composite as substrates for yoghurt production. Pakistan Journal of Nutrition. 2009;8(6):755-8.


18. Mani-López E, Palou E, López-Malo A. Probiotic viability and storage stability of yogurts and fermented milks prepared with several mixtures of lactic acid bacteria. Journal of Dairy Science. 2014;97(5):2578-90.


19. Amakoromo E, Innocent-Adiele H, Njoku H. Physicochemical quality of a yoghurt-like product from African yam bean. Report Opinion. 2012;4(4):58-61.


20. Reitman S, Frankel S. A colorimetric method for the determination of serum glutamic oxalacetic and glutamic pyruvic transaminases. American Journal of Clinical Pathology. 1957;28(1):56-63.


21. Schmidt E, Schmidt F, Möhr J. An improved simple chromatographic method for separating the isoenzymes of malic dehydrogenase and glutamic oxaloacetic transaminase. Clinica Chimica Acta. 1967;15(2):337-42.


22. Juven B, Schved F, Lindner P. Antagonistic compounds produced by a chicken intestinal strain of Lactobacillus acidophilus. Journal of Food Protection. 1992;55(3):157-61.


23. Rochling FA. Evaluation of abnormal liver tests. Clinical cornerstone. 2001;3(6):1-12.


24. Ode O, Asuzu O, Oladele G. The biochemical changes in rats following chronic toxicity with Cassia singueana leaf extract. Journal of Pharmaceutical and Biomedical Sciences. 2011;8:1-4.


25. Berg RD. Translocation of indigenous bacteria from the intestinal tract. Human Intestinal Microflora in Health and Disease. 1983;15:333-52.


26. Johnston DE. Special considerations in interpreting liver function tests. American Family Physician. 1999;59(8):2223.


27. Tilg H, Hotamisligil GS. Nonalcoholic fatty liver disease: cytokine-adipokine interplay and regulation of insulin resistance. Gastroenterology. 2006;131(3):934-45.


28. Weinstein DL, O’Neill BL, Hone DM, Metcalf ES. Differential early interactions between Salmonella enterica serovar typhi and two other pathogenic Salmonella serovars with intestinal epithelial cells. Infection and Immunity. 1998;66(5):2310-8.


29. Schauer DB. Indigenous microflora: Paving the way for pathogens? Current Biology. 1997;7(2):R75-R7.


30. Thirabunyanon M. Biotherapy for and protection against gastrointestinal pathogenic infections via action of probiotic bacteria. Maejo International Journal of Science and Technology. 2011;5(1):108.


31. Guttman JA, Li Y, Wickham ME, Deng W, Vogl AW, Finlay BB. Attaching and effacing pathogen‐induced tight junction disruption in vivo. Cellular Microbiology. 2006;8(4):634-45.


32. Reiff C, Kelly D. Inflammatory bowel disease, gut bacteria and probiotic therapy. International Journal of Medical Microbiology. 2010;300(1):25-33.


33. Nwachukwu E, Umechuruba C. Changes in nutritional values of African yam bean, Sphenostylis stenocarpa (Hochst ex. A. Rich) Harms seeds due to seed-borne fungi. Global Journal of Pure and Applied Sciences. 1997;3(2):141-7.