اثر درون شیشه‌ای تیمارهای سولفات آهن، سولفات روی و سولفات مس بر صفات مورفوفیتوشیمیایی گیاه دارویی Chamaecostus cuspidatus Nak
محورهای موضوعی : گیاهان دارویی
هاجر معتمدی شارک
1
,
محسن ثانی خانی
2
,
عزیزاله خیری
3
,
نیر محمدخانی
4
1 - گروه علوم باغبانی، دانشکده کشاورزی، دانشگاه زنجان، زنجان، ایران
2 - گروه علوم باغبانی، دانشکده کشاورزی، دانشگاه زنجان، زنجان، ایران
3 - گروه علوم باغبانی، دانشکده کشاورزی، دانشگاه زنجان، زنجان، ایران
4 - گروه گیاهان دارویی و معطر، مرکز آموزش عالی شهید باکری میاندوآب، دانشگاه ارومیه، ارومیه، ایران
کلید واژه: ریزمغذی, فلاونوئید, فنل کل, کشت بافت, کلروفیل,
چکیده مقاله :
به‌منظور بررسی اثرات تغییر غلظت عناصر ریزمغذی سولفات آهن، روی و مس محیط کشت پایه موراشیگ و اسکوگ (MS) بر فاکتورهای مورفولوژیکی و فیتوشیمیایی گیاه انسولین، آزمایشی در قالب طرح کاملا تصادفی در آزمایشگاه کشت بافت دانشکده کشاورزی دانشگاه زنجان انجام شد. ریزنمونه‌های گره به مدت 12 هفته در محیط کشت MS با غلظت هورمونی 1 میلی‌گرم در لیتر بنزیل آدنین و 6/0 میلی‌گرم بر لیتر نفتالین استیک اسید رشد کردند. پس از گذشت 12 هفته، ریزنمونه‌‌ها به مدت 4 هفته تحت تیمار سطوح مختلف سولفات آهن (0، 8/27، 7/41، 6/55 میلی‌گرم در لیتر)، سولفات روی (0، 6/8، 2/17، 8/25 میلی‌گرم در لیتر) و سولفات مس (0، 025/0، 05/0، 1/0 میلی‌گرم در لیتر) قرار داده شدند. پس از 4 هفته شاخص‌های رشدی و فیتوشیمیایی مورد ارزیابی قرار گرفت. نتایج آزمایش نشان داد تأثیر تیمارها بر روی صفات مورد مطالعه در سطح احتمال یک درصد معنی‌دار شد. طبق نتایج مقایسه میانگین داده‌ها، سولفات مس با غلظت 1/0 میلی‌گرم در لیتر باعث بیشترین تعداد برگ (33/7 عدد)، ارتفاع اندام هوایی (20/9 سانتی‌متر)، طول ریشه (67/14 سانتی‌متر) وزن تر (26/1 گرم) و خشک اندام هوایی (180 میلی‌گرم)، وزن تر (58/0 گرم) و خشک ریشه (67/146 میلی‌گرم) شد. افزایش قابل توجه کلروفیل a و کل در سولفات روی با غلظت 8/25 میلی‌گرم در لیتر و سولفات مس با غلظت 1/0 میلی‌گرم در لیتر به‌دست آمد. بیشترین کاروتنوئید کل در غلظت 2/17 سولفات روی و غلظت 1/0 میلی‌گرم سولفات مس حاصل شد. در مجموع غلظت 1/0 میلی‌گرم در لیتر سولفات مس بیشترین تاثیر در صفات مورفولوژیکی و رنگدانه‌های فتوسنتزی داشت و سطوح 2/17 میلی‌گرم در لیتر سولفات روی و نیز 05/0 میلی‌گرم در لیتر سولفات مس افزایش قابل توجه‌ای در مقادیر فنل کل و فعالیت آنتی‌اکسیدان داشتند.
In order to investigate the effects of changing the concentration of iron sulfate, zinc sulfate, and copper sulfate micronutrients in MS medium on the morphological and phytochemical factors of the insulin plant, an experiment was conducted based on a completely randomized design in the tissue culture laboratory of the Faculty of Agriculture of University of Zanjan. The nodal explants were grown for 12 weeks in MS culture medium with a hormone concentration of 1 mg/L-1 benzyl adenine and 0.6 mg/L-1 naphthalene acetic acid. After 12 weeks, the explants were treated for 4 weeks with different levels of iron sulfate (0, 27.8, 41.7, 55.6 mg/L-1), zinc sulfate (0, 8.6, 17.2, 25.8 mg/L-1) and sulfate Copper (0, 0.025, 0.05, 0.1 mg/L-1) were placed. After 4 weeks, growth and phytochemical indices were evaluated. The results of the experiment showed that the effect of the treatments on the studied traits was significant at 1% probability level. According to the average data comparison results, copper sulfate with a concentration of 0.1 mg/L-1 caused the highest number of leaves (7.33), shoot height (9.20 cm), root length (14.67 cm) and fresh weight (26.1 g) and shoot dry weight (180 mg), fresh weight (0.58 g) and root dry weight (146.67 mg). A significant increase in chlorophyll a and total chlorophyll was obtained in zinc sulfate with a concentration of 25.8 mg/L-1 and copper sulfate with a concentration of 0.1 mg/L-1. The highest total carotenoid was obtained at the concentration of 17.2 zinc sulfate and 0.1 mg of copper sulfate. In conclusion, the concentration of 0.1 mg/L of copper sulfate had the greatest effect on morphological traits and photosynthetic pigments, and the levels of 17.2 mg/L-1 of zinc sulfate and 0.05 mg/L-1 of copper sulfate had a significant increase in total phenolic content and antioxidant activity.
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