اثر تمرین تناوبی شدید و رژیم غذایی پرپروتئین بر سطوح پلاسمایی آپولیپوپروتئین A1 و B و پروفایل لیپیدی در موشهای صحرایی نر چاق
محورهای موضوعی : فصلنامه زیست شناسی جانوری
مرضیه ثاقب جو
1
,
مهدی علی اکبری
2
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,
هانیه حبیبی سنگانی
3
1 - گروه علوم ورزشی، دانشکده علوم ورزشی، دانشگاه بیرجند، بیرجند، ایران
2 - گروه تربیت بدنی و علوم ورزشی، واحد تهران جنوب، دانشگاه آزاد اسلامی، تهران، ایران
3 - گروه علوم ورزشی، دانشکده علوم ورزشی، دانشگاه بیرجند، بیرجند، ایران
کلید واژه: تمرین ورزشی, رژیم غذایی, مقاومت به انسولین, پروفایل لیپیدی, چاقی,
چکیده مقاله :
چاقی با اختلالات لیپیدی و افزایش قابل توجه بیماریهای قلبی- عروقی همراه است. هدف از مطالعه حاضر بررسی اثر 10 هفته تمرین تناوبی شدید (HIIT) و رژیم غذایی پرپروتئین (HPD) بر سطوح پلاسمایی آپولیپوپروتئین A1 و B و پروفایل لیپیدی در موشهای صحرایی نر چاق بود. 40 سر موش صحرایی نر ویستار چاق در پنج گروه هشتتایی: HIIT، HPD، HPD+HIIT، کنترل چاق A (OC-A) و کنترل چاق B (OC-B) و هشت سر موش به‌عنوان گروه کنترل نرمال (NC) مورد مطالعه قرار گرفتند. گروههای تمرین، پنج روز در هفته به مدت 10 هفته با شدت حدود 90 درصد حداکثر اکسیژن مصرفی روی نوارگردان دویدند. پس از پایان مداخله، نمونه‌گیری خون انجام و سطوح آپولیپوپروتئینهای ApoA1، ApoB و پروفایل لیپیدی در پلاسمای حاصل اندازه‌گیری شد. دادهها با استفاده از آزمون آنالیز واریانس یکطرفه و آزمون تعقیبی توکی آنالیز شدند. سطح پلاسمایی HDL-C بهطور معنیداری در گروه NC بالاتر از گروه OC-A بود (05/0 ˂p )، اما بین سایر گروهها تفاوت معنیداری مشاهده نشد (05/0 ˃p ). نسبتهای TC/HDL-C و LDL-C/HDL-C در گروههای NC و HIIT+HPD در مقایسه با گروه OC-A بهطور معنیداری پایینتر بود (05/0 ˂ p)، در حالیکه بین سایر گروهها تفاوت معنیداری مشاهده نشد (05/0 ˃p ). همچنین تری‌گلیسرید-گلوکز (TyG) نیز بهطور معنیداری در گروههای NC، OC-B، HIIT، HPD و HIIT+HPD پایینتر از گروه OC-A بود (05/0p < ). در سطوح پلاسمایی ApoA1، ApoB، ApoB/ApoA1، تری گلیسرید، کلسترول تام، لیپوپروتئین با چگالی پایین و لیپوپروتئین با چگالی بسیار پایین بین گروهها تفاوت معنیداری مشاهده نشد (05/0 ˃p ). بهنظر می‌رسد انجام HIIT همراه با HPD بهواسطه بهبود پروفایل لیپیدی میتوانند از عوارض متابولیک احتمالی ناشی از چاقی و رژیمهای غذایی پرچرب جلوگیری کنند.
Obesity is associated with lipid disorders and a significant increase in cardiovascular diseases. This study aimed to investigate the effect of 10 weeks of high-intensity interval training (HIIT) and high-protein diet (HPD) on the plasma levels of apolipoprotein A1 and B and lipid profile in obese male rats. 40 obese male Wistar rats in five groups (n = 8): HIIT, HPD, HPD+HIIT, obese control-A (OC-A), and obese control-B (OC-B) and eight rats were also studied as normal control group (NC). Training groups, ran five days a week for 10 weeks with at the about intensity of 90% of maximum oxygen uptake on the treadmill. After the end of the intervention, blood sampling was done and the levels of apolipoproteins ApoA1, ApoB, and lipid profile were measured in plasma. The data were analyzed using one-way analysis of variance and Tukey's post-hoc tests at a significance level of p < 0.05. The plasma level of HDL-C was significantly higher in the NC group than in the OC-A group (p ˂ 0.05), but no significant difference was observed between the other groups (p > 0.05). The TC/HDL-C and LDL-C/HDL-C ratios in the NC and HIIT+HPD groups were significantly lower compared with the OC-A group (p ˂ 0.05), while no significant difference was observed between the other groups (p ˃ 0.05). Also, triglyceride-glucose index (TyG) was significantly lower in the NC, OC-B, HIIT, HPD, and HIIT+HPD groups than in the OC-A group (p < 0.05). No significant difference were observed in the plasma levels of ApoA1, ApoB, ApoB/ApoA1, Triglyceride (TG), Total cholesterol (TC), Low-density lipoprotein (LDL-C), and Very low density lipoprotein (vLDL-C) among the groups (p > 0.05). It seems that doing HIIT along with HPD can prevent possible metabolic complications caused by obesity and high-fat diets by improving the lipid profile.
1. Gonzalez-Gil AM, Elizondo-Montemayor L. The role of exercise in the interplay between myokines, hepatokines, osteokines, adipokines, and modulation of inflammation for energy substrate redistribution and fat mass loss: a review. Nutrients. 2020;12(6):1899.
2. Albarrati AM, Alghamdi MSM, Nazer RI, Alkorashy MM, Alshowier N, Gale N, Effectiveness of low to moderate physical exercise training on the level of low‐density lipoproteins: a systematic review. Biomed Res Int. 2018; 2018:5982980.
3. Alizadeh M, Asad MR, Faramarzi M, Afroundeh R. Effect of eight-week high intensity interval training on omentin-1 gene expression and insulin-resistance in diabetic male rats. Annal Appl Sport Sci. 2017; 5(2):29-36.
4. Garneau P, Glazer S, Jackson T, Sampath S, Reed K, Christou N, et al. Guidelines for canadian bariatric surgical and medical centres: a statement from the canadian association of bariatric physicians and surgeons. Can J Surg. 2022;65(2):E170.
5. Kim DY, Jung SY. Effect of aerobic exercise on risk factors of cardiovascular disease and the apolipoprotein B/apolipoprotein a-1 ratio in obese woman. J Physic Ther Sci. 2014;26(11):1825-1829.
6. Berberich AJ, Hegele RA. A modern approach to dyslipidemia. Endocr Rev. 2022;43(4):611-53.
7. Bailey A, Mohiuddin SS. Biochemistry, High Density Lipoprotein. 2022 Sep 26. In: StatPearls [Internet]. Treasure Island (FL): StatPearls Publishing; 2025; PMID: 31747209.
8. Fernandez ML, Webb D. The LDL to HDL cholesterol ratio as a valuable tool to evaluate coronary heart disease risk. J Am Coll Nutr. 2008; 27(1):1-5.
9. Heidemann BE, Koopal C, Bots ML, Asselbergs FW, Westerink J, Visseren FL. The relation between VLDL-cholesterol and risk of cardiovascular events in patients with manifest cardiovascular disease. Int J Cardiol. 2021;322: 251-7.
10. Duran EK, Aday AW, Cook NR, Buring JE, Ridker PM, Pradhan AD. Triglyceride-rich lipoprotein cholesterol, small dense LDL cholesterol, and incident cardiovascular disease. J Am Coll Cardiol. 2020;75(17): 2122-35.
11. Bhoite R, Chandrasekaran A, Pratti VL, Satyavrat V, Aacharya S, Mane A, et al. Effect of a high‐protein high‐fibre nutritional supplement on lipid profile in overweight/obese adults with type 2 diabetes mellitus: a 24‐week randomized controlled trial. J Nutr Metabol. 2021; 2021:6634225.
12. Kawanishi N, Takagi K, Lee HC, Nakano D, Okuno T, Yokomizo T, et al. Endurance exercise training and high-fat diet differentially affect composition of diacylglycerol molecular species in rat skeletal muscle. Am J Physiol Regul Integr Comp Physiol. 2018;314(6):R892-R901.
13. Huang M, Zheng J, Chen L, You S, Huang H. Role of apolipoproteins in the pathogenesis of obesity. Clinica Chimica Acta. 2023;545:117359.
14. Gianazza E, Zoanni B, Mallia A, Brioschi M, Colombo GI, Banfi C. Proteomic studies on apoB‐containing lipoprotein in cardiovascular research: a comprehensive review. Mass Spectrom Rev. 2023;42(4):1397-423.
15. Du Y., Zhu B., Liu Y., Du Z., Zhang J., Yang W., et al., Association between apolipoprotein B/A1 ratio and quantities of tissue prolapse on optical coherence tomography examination in patients with atherosclerotic cardiovascular disease. Int J Cardiovasc Imaging. 2024;40(3):545-55.
16. Dominiczak MH, Caslake MJ. Apolipoproteins: metabolic role and clinical biochemistry applications. Annal Clin Biochem. 2011;48(6):498-515.
17. Andraski AB, Singh SA, Lee LH, Higashi H, Smith N, Zhang B, et al. Effects of replacing dietary monounsaturated fat with carbohydrate on HDL (high-density lipoprotein) protein metabolism and proteome composition in humans. Arterioscler Thromb Vasc Biol. 2019; 39(11):2411-2430.
18. Cooke AL, Morris J, Melchior JT, Street SE, Jerome WG, Huang R, et al. A thumbwheel mechanism for APOA1 activation of LCAT activity in HDL [S]. J Lipid Res. 2018;59(7):1244-1255.
19. Kostogrys RB, Franczyk-Zarow M, Maslak E, Topolska K. Effect of low carbohydrate high protein (LCHP) diet on lipid metabolism, liver and kidney function in rats. Environmental Toxicology and Pharmacology, 2015;39(2):713-719.
20. Soleimani N, Gallehdari M, Sheikh R. Effect of six weeks of interval training and curcumin consumption on apolipoprotein A and B in diabetic male rats. J Exerc Organ Cross Talk. 2023;3(2):73-80.
21. Hatami M, Roshdi Bonab R, Atashak S. Comparison of the effect of twelve week of concurrent (resistance-aerobic) and high-intensity interval training on the atherogenic, insulin resistance and monocyte chemoattractant protein-1 indices in obese menopausal women. Sport Physiol Manag Invest. 2022,14(1):177-93. [In Persian]
22. Rajar HA, Hashmi MA, Akhtar S, Amin U, John A. The effect of high intensity interval training in reducing the risk of cardiovascular diseases in obese type-I individuals. Allied Med Res J. 2023;1(2): 86-95.
23. Cassidy S, Thoma C, Houghton D, Trenell MI. High-intensity interval training: a review of its impact on glucose control and cardiometabolic health. Diabetologia. 2017; 60(1):7-23.
24. Dolataabadi P, Amirsasan R, Vakili J, The effect of high-intensity interval training on serum asprosin and lipid profile of overweight and obese Women. J Pract Stud Biosci Sport. 2023;11(27):22-33. [In Persian]
25. Nasiri R, Mirzaei B, Faramarzi M, Shirazian F. Comparison of the effect of two moderate and high-intensity endurance and resistance training methods on predictors of cardiovascular diseases and arteriosclerosis in elderly rats. J Police Med. 2023,12(1):0-16. [In Persian]
26. Kouhgardzadeh S, Valipour-Dehnou V, Molanouri-Shamsi M. Effect of high intensity functional training on serum levels of ApoA-I, ApoB and lipid profile in elderly men and women. Feyz Med Sci J. 2022; 26(2):138-146. [In Persian]
27. Kalantar-Zadeh K, Kramer HM, Fouque D. High-protein diet is bad for kidney health: unleashing the taboo. Oxford University Press, 2020; pp:1-4.
28. Zhang X, Sergin I, Evans TD, Jeong SJ, Rodriguez-Velez A, Kapoor D, et al. High-protein diets increase cardiovascular risk by activating macrophage mTOR to suppress mitophagy. Nat Metabol. 2020; 2(1):110-25.
29. Boren J, Chapman MJ, Krauss RM, Packard CJ, Bentzon JF, Binder CJ, et al. Low-density lipoproteins cause atherosclerotic cardiovascular disease: pathophysiological, genetic, and therapeutic insights: a consensus statement from the european atherosclerosis society consensus panel. Eur Heart J. 2020;41(24): 2313-30.
30. Lee JJ, Kim HA, Lee J. The effects of Brassica juncea L. leaf extract on obesity and lipid profiles of rats fed a high-fat/high-cholesterol diet. Nutr Res Pract. 2018; 12(4):298-306.
31. Riyahi V, Morovvati H, KHosravi A. The effect of a period of resistance, endurance and HIIT training on serum glucose index in obese diabetic Wistar rats. J Physiol Mov Health. 2023;3(2):1-12. [In Persian]
32. Chen S, Zhang PZ. Effects of high intensity interval training and crossover point training on blood lipid metabolism in overweight female university students. Chin J Sch Health. 2022;43(10):1495-1499.
33. Rahmati-Ahmadabad S, Broom DR, Ghanbari-Niaki A, Shirvani H. Effects of exercise on reverse cholesterol transport: a systemized narrative review of animal studies. Life Sci. 2019;224:139-48.
34. Ghadery B, Ghazalian F, Hosseini SA, Natanzy HA, Shamsoddini A. Effect of high-intensity interval training with eryngium campestre on lipid profile and glycemic indices in high-fat diet-induced obese rats. Hormozgan Med J. 2020; 24(2):e98982.
35. Abbasi B, Samadi A, Bazgir B. The combined effect of high-intensity interval training and intermittent fasting on lipid profile and peroxidation in wistar rats under high-fat diet. Sport Sci Health. 2020;16:645-652.
36. French WW, Dridi S, Shouse SA, Wu H, Hawley A, Lee SO, et al. A high-protein diet reduces weight gain, decreases food intake, decreases liver fat deposition, and improves markers of muscle metabolism in obese Zucker rats. Nutrients. 2017;9(6):587.
37. Muzaffar H, Faisal MN, Anwar H, Hussain A, Khan JA, Muhammad F, et al., Fish protein intake is a novel dietary approach for managing diabetes‐associated complications in diabetic wistar rat model. Food Sci Nutr. 2021;9(2):1017-24.
38. Pashaei Z, Jafari A, Alivand M. The effect of high intensity interval training on lipid profile and glucose homeostasis in overweight/obese middle-aged women. J Appl Health Stud Sport Physiol. 2020; 6(2):56-64. [In Persian]
39. Da Silva A, Caldas APS, Hermsdorff HHM, Bersch-Ferreira AC, Torreglosa CR, Weber B, et al. Triglyceride-glucose index is associated with symptomatic coronary artery disease in patients in secondary care. Cardiovasc Diabetol. 2019;18:1-8.