بررسی اثر الاژیک اسید بر اختلالات حرکتی و حافظه ناشی از 6- هیدروکسی دوپامین در موش صحرایی نر
محورهای موضوعی : فصلنامه زیست شناسی جانوری
زینب عیدی پور
1
,
مریم رفیعی راد
2
1 - گروه زیست شناسی، واحد ایذه، دانشگاه آزاد اسلامی، ایذه، ایران
2 - گروه زیست شناسی، واحد ایذه، دانشگاه آزاد اسلامی، ایذه، ایران
کلید واژه: حافظه, پارکینسون, حرکت, الاژیک اسید,
چکیده مقاله :
ﺑﻴﻤﺎری ﭘﺎرﻛﻴﻨﺴﻮن ﻳﻚ اﺧﺘﻼل ﻧﻮروﭘﺎﺗﻮﻟﻮژﻳﻚ ﺷﺎﻳﻊ اﺳﺖ ﻛﻪ ﺑـﻪ ﻋﻠﺖ دژﻧﺮاﺳﻴﻮن ﻧﻮرون ﻫﺎی دوﭘﺎﻣینرژﻳﻚ ﺑﺨﺶ ﻣﺘﺮاﻛﻢ ﺟﺴﻢ ﺳﻴﺎه اﻳﺠﺎد ﻣﻲ ﺷﻮد. استرس اکسیداتیو به عنوان یک عامل اصلی ایجاد این بیماری محسوب می شود که موجب مرگ نورون ها می شود. ﺑﺎ ﺗﻮﺟﻪ ﺑﻪ ﺧﺎﺻﻴﺖ آنتی اکسیدانی الاژیک اسید، هدف مطالعه حاضر بررسی اﺛﺮ الاژیک اسید بر اختلالات حرکتی و شناختی در ﻣﺪل حیوانی ﺑﻴﻤﺎری ﭘﺎرﻛﻴﻨﺴﻮن است. چهل سر موش صحرایی نر به پنج گروه هشت تایی تقسیم شدند. به منظور ایجاد مدل حیوانی پارکینسون موش ها، سم عصبی 6- هیدروکسی دوپامین (6-OHDA) را با دوز 8 میکروگرم در 2 میکرولیتر سالین نرمال دارای 01/0 درصد اسید آسکوربیک را درون هسته میانی- قدامی (MFB) سمت چپ مغز دریافت کردند. گروه های درمان الاژیک اسید با دوزهای 10، 25 و 50 میلی گرم بر کیلوگرم را به مدت 14 روز به صورت گاواژ داخل معدی دریافت کردند. یک روز بعد از آخرین گاواژ تست های حرکتی و حافظه از موش ها به عمل آمد. الاژیک اسید باعث بهبود اختلالات حرکتی و حافظه ناشی از پارکینسون بویژه در دوز 50 میلی گرم بر کیلوگرم شد. با توجه به یافته های حاضر در این پژوهش می توان پیشنهاد نمود که الاژیک اسید دارای اﺛـﺮ ﺣﻔﺎﻇﺘﻲ در ﺑﺮاﺑـﺮ 6- ﻫﻴﺪروﻛﺴﻲ دوﭘﺎﻣﻴﻦ در ﻣﺪل ﺗﺠﺮﺑﻲ ﺑﻴﻤﺎری ﭘﺎرﻛﻴﻨﺴﻮن اﺳﺖ.
Parkinson's disease is a prevalent neuropathological disorder induced due to the degeneration of dopaminergic neurons in the dense part of substantia nigra. Oxidative stress is regarded as the main factor of this disease that causes death of neurons. Considering the protective antioxidative property of ellagic acid, the aim of the present study was to investigate the effect of ellagic acid on motor disorders and memory of animal model of Parkinson's disease. Forty male rats were divided into 5 groups. To create an animal model, the rats received 6-OHDA neural poison with dose of 8 µg in 2 µl of normal saline solution with 0.01% of ascorbic acid inside medial forebrain bundle (MFB) on the left side of the brain. The treatment groups received ellagic acid with doses of 10, 25, and 50 mg/kg for 14 d as gastric gavage and tests were conducted on the rats one day after the last gavage. Ellagic acid improves motor and memory disorders motor disorders and memory resulting from Parkinson's disease particularly at doses 50 mg/kg. Considering the present findings, ellagic acid has a protective effect on 6-OHDA in the experimental model of Parkinson's disease.
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