اثر سطوح مختلف منگنز جیره بر فعالیت آنتی اکسیدانی، آنزیم های کبدی و بافت کبد در فیل ماهیان (Huso huso) جوان پرورشی

محورهای موضوعی : فصلنامه زیست شناسی جانوری

فاطمه همتی ¹ , حسین خارا ² , حبیب وهاب زاده رودسری ³ , رضوان اله کاظمی ⁴

1 - گروه شیلات، واحد لاهیجان، دانشگاه آزاد اسلامی، لاهیجان، ایران
2 - گروه شیلات، واحد لاهیجان، دانشگاه آزاد اسلامی، لاهیجان، ایران
3 - گروه شیلات، واحد لاهیجان، دانشگاه آزاد اسلامی، لاهیجان، ایران
4 - سازمان تحقیقات، آموزش و ترویج کشاورزی، انستیتو تحقیقات بین المللی ماهیان خاویاری، رشت، ایران

تاریخ دریافت : 1402/10/26 تاریخ پذیرش : 1402/11/24 تاریخ انتشار : 1403/03/12

کلید واژه: فیل ماهی (Huso huso), منگنز جیره غذایی, فعالیت آنتی اکسیدانی, آنزیم های کبدی, بافت کبد,

چکیده مقاله :

این تحقیق با هدف تعیین اثر مقادیر مختلف منگنز جیره بر فعالیت آنتی اکسیدانی، آنزیم های کبدی و بافت کبد فیل ماهیان (Huso huso) جوان پرورشی، از ماه مهر تا آذر 1401 در مرکز تکثیر و بازسازي ذخاير ژنتیکی ماهیان خاویاری شهید دکتر بهشتی سد سنگر شهرستان رشت در استان گیلان انجام گرفت. بدین منظور 180 قطعه فیل ماهی با میانگین وزن اولیه ماهیان 05/3± 266 گرم پس از 2 هفته سازگاری با محیط پرورشی، در شش تیمار و هر تيمار با سه تكرار، با غلظت های 5 (Mn1)، 10 (Mn2)، 15 (Mn3)، 20 (Mn4) و 25 (Mn5) میلی گرم سولفات منگنز مونوهیدرات (MnSO4H2O) در هر کیلوگرم غذا و تیمار شاهد (Mn0) بدون افزودن مکمل سولفات منگنز در دو ماه انجام شد. در پایان آزمایش از هر تکرار سه قطعه ماهی انتخاب، خونگیری و از کبد آن ها جهت مطالعات بافت شناسی نمونه برداری شد. نتایج فعالیت آنتی اکسیدانی کاتالاز و گلوتاتیون پراکسیداز بین تیمارهای آزمایشی دارای اختلاف معنی دار بود (05/0 > p) و بیشینه میزان آنها در ماهیان تیمار شاهد وجود داشت، ولی سطوح سوپر اکسید دیسموتاز اختلاف معنی داری نداشت (05/0 p)، اما آنزیم آلانین آمینوترانسفراز، فاقد اختلاف معنی دار بود (05/0 < p). همچنین آسیب های بافتی به اشکال مختلف در بافت کبد همه تیمارها، حتی شاهد (آتروفی، رکود صفراوی، دژنرسانس چربی و نکروز سلولی) مشاهده شد. بر اساس نتایج این پژوهش، سطوح 15-10 میلی گرم منگنز جیره توانست سبب بهبود فعالیت های آنتی اکسیدانی، آنزیم های کبدی و نیز کاهش آسیب های بافتی کبد در فیل ماهی جوان پرورشی شد.

چکیده انگلیسی:

This research aims to determine the effect of different amounts of dietary manganese on antioxidant activity, liver enzymes and, liver tissue of rearing young beluga (Huso huso) from October to December 2022 at the Dr. Beheshti Reproduction and Genetic Stock Restoration Center of Sturgeon in Rasht, Guilan province. For this study, 180 pieces of beluga with an average initial weight of 266 ± 3.05 grams underwent a two-week adaptation period in the breeding environment, in six treatment groups and each treatment with three repetitions, with concentrations of 5 (Mn1), 10 (Mn2), 15 (Mn3), 20 (Mn4) and 25 (Mn5) mg of manganese sulfate monohydrate (MnSO4H2O) per kilogram of food and control treatment (Mn0) without adding manganese sulfate supplement were carried out in two months. At the end of each month, three pieces of fish were selected from each repetition, blood was collected and their livers were sampled for histological studies. The results revealed a significant difference in catalase and glutathione peroxidase levels among the experimental treatments (p<0.05) and their maximum amount was the control treatment fish, while superoxide dismutase levels did not differ significantly (p<0.05). Among the liver enzymes, Alkaline-phosphatase and aspartate-aminotransferase had a significant difference between the control treatment and other experimental treatments (p<0.05), but the alanine-aminotransferase enzyme had no significant difference (p<0.05). Also, different forms of tissue damage were observed in the liver tissue of all treatments, even the control (atrophy, biliary stagnation, Fat degeneration and, cellular necrosis). Based on the results of this research, the levels of 10-15 mg of dietary manganese could improve antioxidant activities, liver enzymes and reduce liver tissue damage in breeding young beluga.

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