مقایسه اثر گالبانیک اسید و ژل کیتوزان بر فاکتورهای پیش التهابی در چسبندگی داخل شکمی پس از لاپاراتومی در موش صحرایی
محورهای موضوعی : فصلنامه زیست شناسی جانوریفیروزه سقایی 1 , ملیکا منتظری 2 , مهسا رضوان 3
1 - گروه دامپزشکی، واحد شهرکرد، دانشگاه آزاد اسلامی، شهرکرد، ایران
2 - گروه دامپزشکی، واحد شهرکرد، دانشگاه آزاد اسلامی، شهرکرد، ایران
3 - گروه دامپزشکی، واحد شهرکرد، دانشگاه آزاد اسلامی، شهرکرد، ایران
کلید واژه: لاپاروتومی, چسبندگی داخل شکمی, سایتوکاین‌های التهابی, گالبانیک اسید, کیتوزان,
چکیده مقاله :
چسبندگيهاي محوطه بطني عارضه اي شايع پس از لاپاروتومی (جراحي شكم) بوده كه ميتواند سبب انسداد روده، درد لگن يا نازايي شود. از آنجا که التهاب نقش موثری در القای چسبندگی داخل شکمی دارد و گالبانیک اسید واجد آثار ضد التهاب و ضد سرطان می‌باشد در تحقیق حاضر تاثیر آن بر مهار فاکتورهای پیش التهابی موثر در چسبندگی شکمی پس از لاپاروتومی مانند TNF-α و IL-1β مورد بررسی قرارگرفت. بدين منظور در رت‌های نر نژاد ویستار پس از بیهوشی و باز کردن ناحیه شکم، القای چسبندگی صورت گرفت و در یک گروه 2 سیسی ژل کیتوزان در محلالقای چسبندگی تزریق و شکم بسته شد و در یک گروه به مدت 7 روز گالبانیک اسید با دوز 10 میلی‌گرم/کیلوگرم بصورت داخل صفاقی تزریق شد. در پایان 7 روز، شکم مجدداً بازو وضعیت ایجاد چسبندگی مشاهده و از محل چسبندگي نمونه برداری شد. جهت تعیین میزان بیان ژن فاکتورهای پیش التهابی TNF-α و IL-1β از روش RT-PCR استفاده شد. نتايج مطالعه نشان داد كه تزریق داخل صفاقي گالبانیک اسید همانند ژل کیتوزان به طور مؤثري باعث كاهش بروز چسبندگي داخل شكمي بعد از لاپاروتومي شد. این اثر با کاهش بیان ژن TNF-α و IL-1β در هر دو گروه همراه بود که بیانگر اثر ضد التهاب این دو ماده طبیعی می‌باشد.
Abdominal adhesions are a common complication after laparotomy (abdominal surgery) that can cause intestinal obstruction, pelvic pain and infertility. Since inflammation plays an effective role in induction of intra-abdominal adhesions and galbanic acid has anti-inflammatory and anti-cancer effects, in the present research, its effect on inhibiting of pro-inflammatory factors such as TNF-α and IL-1β, in abdominal adhesions after laparotomy was investigated. For this purpose, after anesthetizing and opening the abdomen, adhesion was induced in male wistar rats and, adhesion was induced in male wistar rats, and in one group, 2 cc of chitosan gel was injected in the adhesion induction site and the abdomen was closed, and in one group, galbanic acid (10mg/ kg) was injected intraperitoneally for 7 days. At the end of 7 days, the abdomen was opened again and the abdominal adhesion was observed and a sample was taken from the adhesion sites. RT-PCR method was used to determine the gene expression of pre-inflammatory factors TNF-α and IL-1β. The results of the study showed that intra-peritoneal injection of galbanic acid like chitosan gel effectively reduced the incidence of intra-abdominal adhesions after laparotomy. This effect was accompanied by a decrease in TNF-α and IL-1β gene expression in both groups, which indicates the anti-inflammatory effect of these two natural substances.
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