اثربخشی هشت هفته تمرین هوازی بر ژنوتیپ های CT و TT پلی‌مورفیسم rs2070744 ژن NOS3 و تغییرات عملکرد هوازی زنان تمرین نکرده
محورهای موضوعی : فصلنامه زیست شناسی جانوریحدیث رحیمی 1 , مانیا روزبیانی 2 , عباس صارمی 3
1 - گروه تربیت بدنی، واحد بروجرد، دانشگاه آزاد اسلامی، بروجرد، ایران
2 - گروه تربیت بدنی، واحد بروجرد، دانشگاه آزاد اسلامی، بروجرد، ایران
3 - گروه تربیت بدنی، واحد اراک، دانشگاه آزاد اسلامی، اراک، ایران
کلید واژه: ژنوتیپ¬های CT و TT, NOS3, تمرین هوازی, چاقی, VO2max,
چکیده مقاله :
پژوهش حاضر با هدف بررسی تأثیر 8 هفته تمرین هوازی بر ژنوتیپهای CT و TT پلی‌مورفیسم rs2070744 ژن NOS3 و تغییرات عملکرد هوازی زنان تمرین نکرده انجام شد. بدین منظور، تعداد 29 زن غیر فعال 30 تا 45 ساله بهصورت تصادفی از زنان داوطلب شهرستان شهریار انتخاب شدند. آزمودنیها 8 هفته تمرین هوازی با تواتر 5 جلسه در هفته و هر جلسه 30 دقیقه با شدت 55 تا 75 درصد ضربان قلب ذخیره انجام داده بهطوریکه در دو هفته اول با 55 تا 65 درصد ضربان قلب بیشینه، در دو هفته دوم با 60 تا 65 درصد ضربان قلب بیشینه و 4 هفته پایانی با 65 تا 75 درصد ضربان قلب بیشینه به تمرین پرداختند. 10 دقیقه برای گرم کردن و 10 دقیقه برای سرد کردن در هر جلسه تمرینی در نظر گرفته شد. برای تعیین میزان VO2max از آزمون 7 مرحلهای بروس، پیش و پس از تمرینات استفاده شد. سپس، از بین آزمودنیهایی که توانستند آزمون مورد نظر را بر اساس انتظار محقق اجرا کنند نمونهگیری بزاقی برای توالییابی DNA برای تعیین ژنوتیپها انجام گرفت. جهت تعیین ژنوتیپ از روش RFLP استفاده شد. نتایج حاصل از بررسی دادهها با استفاده از آزمون t مستقل بررسی شد و نتایج نشان داد که میزان VO2max در مرحله پیش آزمون در زنانی که دارای ژنوتیپ CT بودند بهطور میانگین قبل و بعد از مداخله ورزشی، معنیدار نبوده است (884/0 p =)، میزان VO2max در مرحله پس آزمون در زنانی که دارای ژنوتیپ TT بودند بهطور میانگین قبل و پس از مداخله ورزشی، معنیدار نبوده است (110/0 p =). در این تحقیق نشان داده شد که بهبود معنیدار Vo2max به تفاوتهای ژنوتیپی آنها بستگی نداشت و بین ژنوتیپهای CT و TT پلی‌مورفیسم rs2070744 ژن NOS3 و تغییرات عملکرد هوازی زنان تمرین نکرده چاق بعد از 8 هفته تمرین هوازی رابطه معنیداری مشاهده نشد.
The present study was conducted with the aim of investigating the effect of 8 weeks of aerobic training on the CT and TT genotypes of the rs2070744 polymorphism of the NOS3 gene and the changes in aerobic performance of untrained women. For this purpose, 29 inactive women aged 30 to 45 were randomly selected from volunteer women in Shahriar city. The subjects did 8 weeks of aerobic training with a frequency of 5 sessions per week and each session lasting 30 minutes with an intensity of 55 to 75% of the reserve heart rate, so that in the first two weeks with 55 to 65% of the maximum heart rate, in two weeks Second, they trained with 60 to 65% of the maximum heart rate and the last 4 weeks with 65 to 75% of the maximum heart rate. 10 minutes for warming up and 10 minutes for cooling down were considered in each training session. Bruce's 7-step test was used to determine VO2max before and after training.Then, among the subjects who were able to perform the desired test based on the researcher's expectation, saliva sampling was done for DNA sequencing to determine the genotypes. The RFLP method was used to determine the genotype. The results of the data analysis were analyzed using the dependent t-test and the results showed that the VO2max level in women with CT genotype was not significant before and after the exercise intervention (p = 0.015), the amount of VO2max in women who had the TT genotype was not significant before and after the exercise intervention (p = 0.110). In this research, it was shown that the significant improvement of Vo2max did not depend on their genotypic differences, and no significant relationship was observed between the CT and TT genotypes of the rs2070744 polymorphism of the NOS3 gene and the changes in aerobic performance of obese untrained women after 8 weeks of aerobic training.
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