بررسی پلی مورفیسم C23237T ژن Cyp3a4 در افراد سالم غرب مازندران از نظر سن و جنسیت

محورهای موضوعی : فصلنامه زیست شناسی جانوری

فاطمه حیدریان ¹ (گروه زیست شناسی، واحد دامغان، دانشگاه آزاد اسلامی، دامغان، ایران)
ویدا حجتی ² (گروه زیست شناسی، واحد دامغان، دانشگاه آزاد اسلامی، دامغان، ایران)
رضا گلیجانی مقدم ³ (گروه زیست شناسی، واحد تنکابن، دانشگاه آزاد اسلامی، تنکابن، ایران)

تاریخ دریافت : 1401/06/23 تاریخ پذیرش : 1401/10/18 تاریخ انتشار : 1402/06/01

کلید واژه: جنسیت, ژنوتیپ, پلی‌مورفیسم, Cyp3a4, ARMS-PCR,

چکیده مقاله :

Cyp3a4 Cyp3a4 (سیتوکروم P450 3A4) یک آنزیم مهم در بدن است که عمدتاً در کبد و روده یافت می شود. مولکول های آلی کوچک خارجی مانند سموم یا داروها را اکسید می کند تا بتوان آنها را از بدن خارج کرد. از آنجا که مطالعات چندانی روی پلی مورفیسم های این ژن صورت نگرفته این مطالعه به منظور بررسی پلی مورفیسم C23237T ژن Cyp3a4در افراد سالم مذکر و مونث هفت ماهه تا 79 ساله در غرب مازندران انجام شد. در این تحقیق، خون تام از افراد مذکر و مونث سالم شهرهای غرب استان مازندران دریافت شد. برای هر فرد اطلاعات مربوط به سن، جنس، سابقه فامیلی، مصرف الکل و دخانیات ثبت شد. در این بررسی از تکنیک ARMS-PCR استفاده شد. تجزیه و تحلیل داده ها با استفاده از نرم افزار SPSS 2016 و آزمون مربع کای انجام شد. در جمعیت مورد مطالعه 50 نفر مونث و 38 نفر مذکر بودند. نتایج نشان داد در جنس مونث، 33 نفر دارای ژنوتیپ هتروزیگوت با درصد فراوانی 66 درصد، 11 نفر دارای ژنوثیپ هموزیگوت غالب با درصد فراوانی 22 درصد، 5 نفر دارای ژنوتیپ هموزیگوت مغلوب با درصد فراوانی 10 درصد و 1 نفر فاقد جهش با درصد فراوانی 2 درصد بودند. در جنس مذکر، 22 نفر دارای ژنوتیپ هتروزیگوت با فراوانی 89/57 درصد، 5 نفر دارای ژنوتیب هموزیگوت غالب با درصد فراوانی 15/13 درصد، 8 نفر دارای ژنوتیپ هموزیگوت مغلوب با درصد فراوانی 05/21 درصد و 3 نفر فاقد جهش با درصد فراوانی 89/7 درصد بودند. از نظر جنسیت هم در جنس مونث و هم در جنس مذکر ژنوتیپ هتروزیگوت بالاترین درصد فراوانی را به خود اختصاص داد. همچنین بین پلی مورفیسم C23237Tژن Cype3a4 با سن افراد (81/0 = p) و با جنسیت افراد (845/0 = p) ارتباط معناداری وجود ندارد.

چکیده انگلیسی:

CYP3A4 gene is located on chromosome 7 and the largest number of substrates among cytochrome p450 enzymes belongs to it. The CYP3A4 gene is present in the endoplasmic reticulum of all tissues except the brain, although the main place of accumulation of its protein is in the liver and prostate. Because there have not been many studies on the polymorphisms of this gene, especially in Iran, this study was conducted in order to investigate the C23237T polymorphism of Cyp3a4 gene in 103 healthy male and female subjects aged 7 months to 79 years-old in the west of Mazandaran. In this research, whole blood was collected from healthy male and female individuals in the western cities of Mazandaran province. For each person, information related to age, sex, family history, alcohol and tobacco consumption, and the patient's medication were recorded. ARMS-PCR technique was used in this study. Data analysis was done using SPSS 2016 software and chi square test. The significant level of polymorphism relationship with gender and age of people was considered as p < 0.05. The results of the research showed that 70 people had the heterozygous status of the mutated AG genotype, the frequency of which was 67.96%. There was no significant relationship between genotype frequency and patients' age. Also, there is no significant relationship between C23237T polymorphism of Cype3a4 gene with age (p = 0.81) and gender (p = 0.845). According to the results of this research, it can be considered that people in any age group and with any gender have the same probability of contracting the disease.

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