بررسی مقایسه ای عصاره هیپوفیز، LHRH و تیروکسین بر استروئیدهای جنسی، هیستوشیمی بافت تخمدان و اسیدهای چرب مولد مولد ماده روهو (Labeo rohita)

محورهای موضوعی : فصلنامه زیست شناسی جانوری

احسان اسلامی زاده ¹ (گروه شیلات، واحد اهواز، دانشگاه آزاد اسلامی، اهواز، ایران)
حدیده معبودی ² (گروه شیلات، واحد اهواز، دانشگاه آزاد اسلامی، اهواز، ایران)
لاله رومیانی ³ (گروه شیلات، واحد اهواز، دانشگاه آزاد اسلامی، اهواز، ایران)
مهران جواهری بابلی ⁴ (گروه شیلات، واحد اهواز، دانشگاه آزاد اسلامی، اهواز، ایران)
مژده چله مال دزفول نژاد ⁵ (گروه شیلات، واحد اهواز، دانشگاه آزاد اسلامی، اهواز، ایران)

تاریخ دریافت : 1400/04/14 تاریخ پذیرش : 1400/05/15 تاریخ انتشار : 1401/06/01

کلید واژه: هورمون, اسیدهای چرب, تیروکسین, هیپوفیز, LHRH, ماده روهو (Labeo rohita),

چکیده مقاله :

این مطالعه با هدف بررسی اثر مقایسه ای هورمون های هیپوفیز، LHRH و تیروکسین بر شاخص های هورمونی و اسیدهای چرب تخمدان ماهی مولد ماده روهو (Labeo rohita) انجام شد. هورمون تیروکسین با دوزهای 2، 10 و 50 میکروگرم به ازای هر گرم وزن بدن ماهی مولد در یک تزریق در نظر گرفته شد. در زمان بین تزریق اول و دوم، بعد از تزریق دوم و بعد از تخم کشی سطح هورمون های استرادیول، تستوسترون، 17- آلفا هیدروکسی پروژسترون، کورتیزول و LH بالاترین سطح خود را به ترتیب در هیپوفیز و تیمار دریافت کننده دوز 50 میکروگرم وزن بدن و کمترین میزان خود را در تیمارهای دریافت کننده LHRH و شاهد داشتند (05/0p <). در بین تیمارهای دریافت کننده تیروکسین، افزایش سطح تیروکسین توانست هورمون های محرک فرآیند رسیدگی را به شکل معنی داری افزایش دهد. تیمار دریافت کننده هیپوفیز و تیمار تیروکسین دریافت کننده 50 میکروگرم با مقدار 70/16 و 99/15 درصد وزن خشک بالاترین میزان پروتئین تخمدان و دو تیمار LHRH و شاهد کمترین سطح پروتئین تخمدان را داشتند. تیمار های دریافت کننده تیروکسین به شکل معنی داری مقدار بالاتری چربی در مقایسه با هیپوفیز، LHRH و شاهد داشت. اسیدهای چرب اشباع اسید چرب C16:0 با مقادیر بین 44/28-16/21 درصد وزن خشک، اسید چرب C24:0 با محدوده ی 60/19-15/16 درصد وزن خشک و اسید چرب C22:0 17/11-34/7 درصد وزن خشک فراوانترین اسیدهای چرب اشباع بودند. بالاترین میزان اسیدهای چرب اشباع (ΣSUF) با مقادیر 15/63 و 73/58 درصد وزن خشک به تیمار دریافت کننده هیپوفیز و تیمار دریافت کننده 50 میکروگرم و کمترین میزان این پارامتر با 55/54 و 14/50 درصد وزن خشک به تیمار شاهد و دریافت کننده LHRH تعلق داشت. دو تیمار هیپوفیز و تیمار دریافت کننده 50 میکروگرم وزن بدن تیروکسین، مجموع اسید چرب امگا 6 (Σω6) و 3 (Σω3) بالاتری داشتند (05/0p <). هورمون هیپوفیز و به دنبال آن هورمون تیروکسین با دوز 50 میکروگرم در مقایسه با دوزهای پایین تیروکسین و نیز LHRH کارایی بالاتری در افزایش سطح هورمون های جنسی دخیل در فرآیند رسیدگی جنسی و نیز ترکیب اسیدهای چرب تخمدان ماهی مولد روهو دارند.

چکیده انگلیسی:

This study aimed at assessing the comparative effect of pituitary hormones, LHRH, and thyroxine on hormonal indices and ovarian fatty acids of Labeo rohita. Blood samples were taken before injection, between the first and second injection, after the second injection and after ovulation, using a 5 ml plastic syringe from the tail stem. According to the measurement results, at the time between the first and second injections, after the second injection and after ovulation, the levels of estradiol, testosterone, 17-alpha hydroxy progesterone, cortisol and LH reached their peak levels in the pituitary and treatment receiving 50 μg dose had the lowest body weight in treatments receiving LHRH and control (P <0.05). Among thyroxine-receiving treatments, increasing thyroxine levels could significantly increase the hormones stimulating the maturation process (P <0.05). Pituitary receiving treatment and thyroxine receiving 50 μg per body weight had the highest amount of ovarian protein (P <0.05), with 16.70 and 15.99% dry weight and two LHRH and control treatments had the lowest ovarian protein level (P <0.05). Regarding fat, thyroxine-receiving treatments had a significantly higher amount compared to pituitary, LHRH, and control (P <0.05). The highest amount of saturated fatty acids (ΣSUF) with values ​​of 63.15 and 58.73% of dry weight to the pituitary receiving treatment and receiving 50 μg per body weight and the lowest amount of this parameter with 54.55 and 50.14% dry weight belonged to the control and LHRH recipient, respectively. C17: 1 and C18: 1n9c fatty acids in the range of 3.43-4.77 and 3.28-5.40% of dry weight and C24: 1n9 fatty acids in the range of 0.03-0.18% by dry weight had the highest and lowest levels of unsaturated fatty acids (ΣPUF), respectively. Both pituitary and 50 μg treatments of thyroxine had higher total omega-6 (Σω6) and 3 (Σω3) fatty acids (P <0.05). The results of the present study revealed that pituitary hormone followed by thyroxine at a dose of 50 μg per body weight compared to low doses of thyroxine and LHRH were more effective in increasing the level of sex hormones involved in the process of sexual maturation and the combination of acids. 

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