تأثیر یک دوره تمرین تناوبی با شدت بالا بر بیان سطوح پلاسمایی ASTو ALT در بافت قلبی موش‌های صحرایی نر نژاد ویستار مبتلا شده به کبد چرب

محورهای موضوعی : فصلنامه زیست شناسی جانوری

الهام میرزاییان ¹ , مانیا روزبیانی ² , حسین شیروانی ³

1 - گروه تربیت بدنی و علوم ورزشی، واحد بروجرد، دانشگاه آزاد اسلامی، بروجرد، ایران
2 - گروه تربیت بدنی و علوم ورزشی، واحد بروجرد، دانشگاه آزاد اسلامی، بروجرد، ایران
3 - مرکز تحقیقات فیزیولوژی ورزشی، پژوهشکده سبک زندگی، دانشگاه علوم پزشکی بقیه الله (عج)، تهران، ایران

تاریخ دریافت : 1401/06/03 تاریخ پذیرش : 1401/08/01 تاریخ انتشار : 1402/03/01

کلید واژه: کبد چرب, ALT, آنزیمAST, ورزشHIIT,

چکیده مقاله :

تمرینات تناوبی پرشدت (HIIT)، روشی تمرینی است که با توجه به زمان کوتاه و حجم کم، در سال های اخیر به عنوان تمرین با آثاری به مراتب موثرتر از تمرینات سنتی هوازی معرفی شده است. این مطالعه با هدف بررسی تأثیر یک دوره تمرین تناوبی شدید بر بیان سطوح پلاسمایی آنزیم AST و ALT در بافت قلبی رت های نر مبتلا به کبد چرب (استئاتوزیس) انجام شد. نمونه آماری شامل 48 عدد رت نر نژاد ویستار (در 2 مدل سالم و مبتلا به کبد چرب) با سن 5 هفته و وزن 200-250 بوده و شرایط یکسان (12:12 تاریکی و روشنایی، دمای 2±22 درجه سانتیگراد، رطوبت 50-60 درصد رطوبت 51 درصد)، در قفس نگهداری شدند با استفاده از ارزیابی آنزیم SGOT با کیت‌های تشخیصی شرکت ZiestChem انجام شد. تجزیه‌و تحلیل داده‌ها با آزمون‌های t مستقل، تحلیل واریانس یک‌طرفه و آزمون تعقیبی توکی انجام گرفت و داده ها با نرم افرار SPSS نسخه 22 مورد تجزیه و تحلیل قرار گرفت. با توجه به نتایج، میزان بیان آنزیم AST.ALT در رت های گروه بیمار نسبت به رت های گروه سالم افزایش یافته است (05/0 > p) که با توجه به سطح معنی‌داری به‌دست‌آمده، این اختلاف معنی‌دار بود. بنظر می رسد که یک دوره تمرین تناوبی شدید بر بیان سطوح پلاسمایی آنزیم AST,ALT در بافت قلبی رت های نر مبتلابه کبد چرب (استئاتوزیس) تاثیر دارد و می تواند به عنوان مارکری برای پیشگیری و درمان بیماری های قلبی و عروقی، کبد چرب ،چاقی همچنین دیابت استفاده شود.

چکیده انگلیسی:

High-intensity interval training (HIIT) is a kind of exercise training that is suggested for individuals with sufficient time for regular training.  This study aimed to evaluate the effect of a period of intense intermittent training on the expression of plasma levels of AST, and ALT enzyme in the cardiac tissue of male rats with fatty liver (steatosis model). The statistical sample consisted of 48 male Wistar rats (in2 healthy models with fatty liver) aged 5 weeks and weighing 200-250 and in the same conditions (12:12 darkness and light, temperature 22 ± 2 degrees Celsius (50-60%) humidity (51% humidity) were kept in cages. Gene expression was assessed using Real-timePCR and AST, and ALT enzyme was assessed using ZiestChem diagnostic kits. Data were analyzed by independent t-test, one-way analysis of variance, and Tukey's post hoc test, and the data were analyzed by SPSS software version 22. According to the results, the expression of ASTandALT enzymes in the rats of the patient group increased compared to the rats of the healthy group (p < 0.05), which according to the level of significance It was significant. It seems that a period of intense intermittent training affects the expression of plasma levels of ASTand ALT enzymes in the cardiac tissue of male rats with fatty liver (steatosis model) and can serve as a marker for the prevention and treatment of diseases. Use cardiovascular, fatty liver, obesity as well as diabetes. Keywords:, AST, ALT anzem, HIIT exercise

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